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EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFLAG/HA-TNRC6B

Plasmid

#10979

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6B (TNRC6B Human)

Tags

FLAG and HA

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-DDR2

Plasmid

#23897

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR2 (DDR2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pDONR223_HSP90B1_WT

Plasmid

#82130

Purpose

Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HSP90B1 (HSP90B1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pUC8 mouse IFN-gamma

Plasmid

#10955

Depositor

Howard Young

Insert

interferon-gamma (Ifng Mouse)

Use

Cloning vector

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-LRPPRC

Plasmid

#23521

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LRPPRC (LRPPRC Human)

Use

Gateway Cloning

Mutation

G246A; deleted E579

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo DEST (w18-1)

Plasmid

#17390

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pPS2991

Plasmid

#8931

Depositor

Pamela Silver

Publication

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NDR2 (STK38L Human)

Tags

FLAG

Expression

Mammalian

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Publication

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUBC_V5_MS2-mCherry

Plasmid

#68420

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MS2

Use

Synthetic Biology

Tags

V5 and mCherry

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFRT/TO/FLAG/HA-DEST TNRC6C

Plasmid

#19885

Depositor

Thomas Tuschl

Publication

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

FLAG/HA

Expression

Mammalian

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDestattB (pCM268)

Plasmid

#68313

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

Depositor

Christian Mosimann, Leonard Zon

Publication

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ABE8.8-m

Plasmid

#136294

Purpose

expresses ABE8.8-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.8-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Expression

Mammalian

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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FLAG-Rheb (Q64L)-pcw107

Plasmid

#64607

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Mutation

Q64L

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Publication

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 (MOV10 Human)

Tags

myc

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MED12_pLX307

Plasmid

#98350

Purpose

Lentiviral expression of MED12

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

MED12 (MED12 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R1392Q

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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