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pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mCol.2lox4F2A targeting construct

Plasmid

#25795

Depositor

Rudolf Jaenisch

Article

Carey et al Nat Methods. 2010 Jan . 7(1):56-9.

Insert

loxP-Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc-loxP

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

Plasmid

#18017

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V ECFP-W67G

Tags

ECFP-W67G and Venus

Expression

Mammalian

Mutation

mglB-D154A; non-functional ECFP

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRBBm69

Plasmid

#48116

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - sacB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Tags

tagRFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEntr-SRFflbio

Plasmid

#32971

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

SRF (SRF Human)

Use

Gateway

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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YFP-tTA-ER-C3

Plasmid

#134568

Purpose

Expresses a YFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-A

Plasmid

#44130

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pLOX-EWgfp

Plasmid

#12242

Depositor

Didier Trono

Article

Salmon et al Blood. 2000 Nov 15. 96(10):3392-8.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

EF1a promoter is upstream of GFP. LoxP in 3'…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V Venus-Y67G

Plasmid

#18016

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13V Venus-Y67G

Tags

ECFP and Venus-Y67G

Expression

Mammalian

Mutation

mglB-D154A; non-functional Venus

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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NCOR2

Plasmid

#40737

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NCOR2 (NCOR2 Human)

Tags

6xHis TEV

Expression

Bacterial

Mutation

Residues 615 to 685 of the SANT2 domain of Nuclea…

Available Since

Oct. 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pT2KXIGdeltaIn-MCS-birA-EGFP

Plasmid

#58377

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA EGFP

Tags

EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Cas9m2-VP64

Plasmid

#47317

Purpose

Cas9m2 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pXCX.Syn.EGFP

Plasmid

#38054

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

Synapsin (human SYN1)

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pFT1.2

Plasmid

#46140

Depositor

Wenbiao Chen

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pFT1.1

Plasmid

#46139

Depositor

Wenbiao Chen

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos7_AmpR

Plasmid

#119150

Purpose

Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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