167,318 results

1. qTAG-N-Puro-mScarlet

   Plasmid

   #207689

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. lenti-sgRNA hygro

   Plasmid

   #104991

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-TetO-hNGN2-Puro

   Plasmid

   #79049

   Purpose

   Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Promoter

   TetO

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVD442

   Plasmid

   #11074

   Depositor

   Michael Donnenberg, James Kaper

   Article

   Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

   Insert

   sacB (sacB Bacillus subtilis)
   

   Expression

   Bacterial

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. RARS_pNIC-Bio3

   Plasmid

   #153055

   Purpose

   Expression construct for producing Avi tagged Arginyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   RARS (RARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-LgBit

   Plasmid

   #234738

   Purpose

   Mammalian expression of large NanoBit fragment (LgBit)

   Depositor

   Nevin Lambert

   Article

   Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

   Insert

   LgBit
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATG9A-EGFP

   Plasmid

   #210367

   Purpose

   Expresses ATG9A fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCK-Drosha(UniProt)-FLAG

   Plasmid

   #164690

   Purpose

   Express Drosha-FLAG in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   Drosha (DROSHA Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRADZ1

    Plasmid

    #47698

    Purpose

    General cloning vector for use in E. coli or D. radiodurans with lacZ

    Depositor

    Mary Lidstrom

    Article

    Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    repU

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-EGFP

    Plasmid

    #89871

    Purpose

    EGFP reporter vector for iTango system

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. nls-transposase-nls-pA in pT3TS-Dest

    Plasmid

    #140873

    Purpose

    Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nls-transposase-nls (Tol2)
    

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP12360 - pAAV-AiE0519h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2360)

    Plasmid

    #223986

    Purpose

    AiE0519h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-NL1B

    Plasmid

    #15261

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 with insert B (Nlgn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES-mtGFP

    Plasmid

    #45053

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Expression

    Yeast

    Promoter

    GAL

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-scFvGCN4sfGFP-VP64-GB1

    Plasmid

    #141417

    Purpose

    Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

    Depositor

    Izuho Hatada

    Article

    Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

    Insert

    scFvGCN4sfGFP-VP64-GB1
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLinker-2xTy-HXGPRT-LoxP

    Plasmid

    #86664

    Purpose

    CRISPR tagging with 2xTy in Toxoplamsa gondii

    Depositor

    David Sibley

    Insert

    Linker-2xTy-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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