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Showing: 5301 - 5320 of 35165 results

  3. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  6. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  7. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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  8. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Promoter
    constitutive
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  11. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

    Plasmid
    #133348
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_blaA and sgRNA_cpaA
    Use
    CRISPR
    Promoter
    constitutive
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133348-plasmid-map-sequence-id-267409

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  13. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid
    #111922
    Purpose
    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_1.2μ
    Tags
    CoxVIIIx2
    Expression
    Mammalian
    Mutation
    E67D, E104D, D133E at CaM
    Promoter
    CMV
    Available Since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111922-plasmid-map-sequence-id-214013

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  17. His8:MBP-tev-Ala-eK-Flv

    Plasmid
    #83393
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83393-plasmid-map-sequence-id-174595

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  18. His8:MBP-tev-Ile-eK-Flv

    Plasmid
    #83392
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83392-plasmid-map-sequence-id-174594

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  19. His8:MBP-tev-Tyr-eK-Flv

    Plasmid
    #83391
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83391-plasmid-map-sequence-id-174593

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Showing: 5301 - 5320 of 35165 results