We narrowed to 18,003 results for: Uty

1. pTwist-SARS-CoV-2 Spike-deltaC18 A222V (GTG)

   Plasmid

   #212329

   Purpose

   Encodes SARS-CoV-2 spike A222V for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike A222V (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   A222V

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. H2B-GFP-Aurora B KD

   Plasmid

   #184043

   Purpose

   Expresses H2B-GFP fused to Aurora B kinase dead

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   AURKB (AURKB Human)
   

   Tags

   EGFP and H2B

   Expression

   Mammalian

   Mutation

   Changed Lysine 65 to Arginine

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Repo-Man-1-890

   Plasmid

   #184272

   Purpose

   Expresses C-terminally truncated GFP-RepoMan

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   truncated Repo-Man after aminoacid 893

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-RepoMan-S893D

   Plasmid

   #204995

   Purpose

   Expresses shRNA resistant GFP-RepoMan S893D Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Serine 893 to Aspartic acid

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHES7-NLuc-2A-tdTomato

   Plasmid

   #204348

   Purpose

   Donor vector to tag endogenous pig HES7 locus

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   NLuc-2A-tdTomato
   

   Tags

   NanoLuc and tdTomato

   Expression

   Mammalian

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28 mOct4-POU

   Plasmid

   #206393

   Purpose

   Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBT9-FHIP

   Plasmid

   #198527

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   FHIP (FHIP1B Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   silent mutations in codons 353 and 651 as well as…

   Promoter

   ADH1

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RAT +D122H

   Plasmid

   #187426

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Expression

   Insect

   Mutation

   changed aspartic acid at 129 for histidine

   Promoter

   PH and p10

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. RAT +R111E

   Plasmid

   #187425

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Expression

   Insect

   Mutation

   changed arginine at 118 to glutamic acid

   Promoter

   PH and p10

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CIRC

    Plasmid

    #186343

    Purpose

    Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

    Depositor

    Giovanni Blandino

    Article

    Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

    Insert

    VEGFA (VEGFA Human)
    

    Expression

    Mammalian

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSNA-c029

    Plasmid

    #175471

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    L281R, H288A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Tac-STC(5A)

    Plasmid

    #162491

    Purpose

    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid

    #155063

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid

    #155061

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid

    #155060

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR_P4-P1R:PER36modp

    Plasmid

    #128431

    Purpose

    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    PEROXISADE36 (AT3G50990 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Mutation

    Premature stop codons introduced into the Xero1 (…

    Promoter

    PEROXISADE36

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

    Plasmid

    #105022

    Purpose

    Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Apc (Apc Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

    Plasmid

    #105032

    Purpose

    Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    gRNA targeting Hgs (Hgs Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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