We narrowed to 150,834 results for: ins

1. pRK5-HA-YFP-Raptor K840R

   Plasmid

   #204418

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 840 to Arginine (K840R)

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-YFP-Raptor K1097R

   Plasmid

   #204417

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 1097 to Arginine (K1097R)

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBLADE_ONLY_C

   Plasmid

   #168050

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Insert

   Blue light-inducible AraC dimers in Escherichia coli
   

   Expression

   Bacterial

   Promoter

   J23101**

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher002-oTet

   Plasmid

   #124663

   Purpose

   Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTet
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyCatcher002-oTri

   Plasmid

   #124662

   Purpose

   Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oTri
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNP1 sfGFP toehold switch

   Plasmid

   #107355

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold 7 + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTM1_VP35_ZEBOV

   Plasmid

   #68121

   Purpose

   Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   VP35 ZEBOV (VP35 )
   

   Use

   T7

   Promoter

   T7

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-N-I-N-CAAX

   Plasmid

   #41686

   Purpose

   Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   Dronpa145N-IntersectinDH-Dronpa145N-CAAX
   

   Tags

   CAAX box

   Expression

   Mammalian

   Mutation

   K145N on Dronpa

   Promoter

   CMV

   Available Since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NBid-PhoCl2c-CBid

    Plasmid

    #164051

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl2c-CBid
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTM1_VP30_ZEBOV

    Plasmid

    #69119

    Purpose

    Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP30 ZEBOV (VP30 )
    

    Use

    T7

    Promoter

    T7

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. raACR-mCerulean3-pAAV2-hSyn

    Plasmid

    #204966

    Purpose

    Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    AAV

    Tags

    mCerulean3

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28sumo-hRig-I DCARDS

    Plasmid

    #210828

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

    Insert

    hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CT534

    Plasmid

    #233682

    Purpose

    Red light-inducible Vcre-C; Vcre-278C-PhyA

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    Vcre-278C-PhyA
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIE2-DBD

    Plasmid

    #21602

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    GAL4 DBD (GAL4 Budding Yeast)
    

    Use

    Gateway destination vector

    Tags

    3xHA and Gateway destination cassette

    Expression

    Insect

    Available Since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS-PesaRlux

    Plasmid

    #47640

    Purpose

    wt esaR promoter in pCS26

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    PesaR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PesaR

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-RT002-LV

    Plasmid

    #163382

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEGFRtm (LV)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 3xF-GAR1

    Plasmid

    #126873

    Purpose

    Expresses 3xFlag GAR1

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    GAR1 (GAR1 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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