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pHR-SIN-PTEN-L186V

Plasmid

#30379

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L186V (PTEN Human)

Expression

Mammalian

Mutation

L186V

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L42R

Plasmid

#30375

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L42R (PTEN Human)

Expression

Mammalian

Mutation

L42R

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-G20E

Plasmid

#30373

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-G20E (PTEN Human)

Expression

Mammalian

Mutation

G20E

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-S10N

Plasmid

#30371

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-S10N (PTEN Human)

Expression

Mammalian

Mutation

S10N

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-T383D

Plasmid

#30381

Depositor

Todd Waldman

Publication

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-T383D (PTEN Human)

Expression

Mammalian

Mutation

T383D

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
huCof K22Q-mRFP

Plasmid

#245965

Purpose

Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Publication

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q

Promoter

CMV

Available since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 K26/27Q IRES Cherry

Plasmid

#228112

Purpose

Lentiviral expression of human INTS4 K26/27Q

Depositor

Tom Misteli

Publication

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Lysine 26 and 27 to Glutamine

Promoter

EF 1alpha

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP14-1535-1601-Hygro

Plasmid

#238100

Purpose

Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP14 (PARP14 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1535-1601 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DDHD2-30-202-Hygro

Plasmid

#238101

Purpose

Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DDHD2 (DDHD2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 30-202 only

Promoter

EF1alpha

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Publication

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE

Plasmid

#224936

Purpose

pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Depositor

Alexander Lohman

Publication

Aggarwal et al Nat Commun. 2026 Jun 22. doi: 10.1038/s4

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

Ef1a

Available since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M02-412

Plasmid

#225665

Purpose

Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

Depositor

Dominic Esposito

Publication

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

SORE6-mCMVp and WPRE-SV40p

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Publication

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-AERRIE

Plasmid

#204023

Purpose

Lentiviral vector expressing human long non-coding RNA AERRIE (linc01013)

Depositor

Reinier Boon

Publication

Pham et al Front Cell Dev Biol. 2021 Jan 11;8:61907

Insert

long non-coding RNA AERRIE (LINC01013 Human)

Use

Lentiviral

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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