We narrowed to 6,966 results for: mag

1. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

   Plasmid

   #85072

   Purpose

   Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor constitutively active (Q63L) (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q63L

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG106 Lyn11-FAST

   Plasmid

   #130721

   Purpose

   Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   lyn11-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-Ribo-jGCaMP8m

   Plasmid

   #167574

   Purpose

   DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV1

   Insert

   Ribo-GCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACTB-(A)1x

   Plasmid

   #112055

   Purpose

   ACTB mRNA tagged with 1 copy of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   1 copy of Riboglow tag (variant A) after stop cod…

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-NDUFA5-mVenus

   Plasmid

   #185646

   Purpose

   NDUFA5 Complex I sub-unit with C-terminus mVenus tag

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   NDUFA5 (NDUFA5 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-puro/Flag PD-L1 N219Q

   Plasmid

   #121452

   Purpose

   expression of huamn PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N219Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19_GNB1L_dTAG

   Plasmid

   #224378

   Purpose

   Generates GNB1L dTAG C terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   dTAG

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-CTNNB1-S45

    Plasmid

    #164587

    Purpose

    Expresses a gRNA that overlaps the S45 codon of human CTNNB1

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-BRPF3

    Plasmid

    #65383

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRPF3 (BRPF3 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCDH-puro/Flag PD-L1 N200Q

    Plasmid

    #121451

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N200Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT-TO-RPB1-His K1268R CRres si2,4R

    Plasmid

    #139405

    Purpose

    Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1268R

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNK115

    Plasmid

    #219745

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D37E, V181I, S323M, M385K

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG156 Mito-FAST

    Plasmid

    #130723

    Purpose

    Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Mito-FAST
    

    Use

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCrisprV2 sgRNA hPER2c-term #1

    Plasmid

    #179454

    Purpose

    Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    sgRNA targeting human PER2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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