We narrowed to 9,299 results for: yeast expression

1. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. BB2_AB

   Plasmid

   #98534

   Purpose

   empty BB2 for transcription unit cloning

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT1

   Plasmid

   #98299

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGSKU

   Plasmid

   #72243

   Purpose

   Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   kanMX4
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3259 pCMV hUBC9 mt HA

   Plasmid

   #14439

   Depositor

   Peter Howley

   Article

   Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

   Insert

   UBC9 C93S (UBE2I Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Cys93Ser mutation (TGC changed to AGC)

   Promoter

   CMV

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETCON-HtsptLB12v.3

   Plasmid

   #45121

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   HtsptLB12v.3
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGSHU

   Plasmid

   #72244

   Purpose

   Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   hyg
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPMVAd3

   Plasmid

   #98298

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBJ1806 pRS413-COF1RFP

   Plasmid

   #37101

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Promoter

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS426-GFP-Osh2-PH

    Plasmid

    #71373

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh2-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa 277-398

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCTcon2-5INS_EV3

    Plasmid

    #102293

    Purpose

    Yeast Surface Display of 5Ins_16_EV3

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Evolved Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG23A

    Plasmid

    #102884

    Purpose

    Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS426-GFP-Osh1-PH

    Plasmid

    #71372

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Osh1-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa267-388

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJ1807 pRS415-COF1RFP

    Plasmid

    #37102

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Promoter

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pBJ1808 pRS416-COF1RFP

    Plasmid

    #37103

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Promoter

    Available Since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pGAD-Atg17

    Plasmid

    #121386

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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