161,398 results

1. pRJPaph-mChe

   Plasmid

   #67034

   Purpose

   Plasmid for Paph-mCherry integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   mCherry
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lamp1_mStayGold

   Plasmid

   #221902

   Purpose

   Expresses mStayGold fused to Lamp1

   Depositor

   Janelia Integrative Imaging

   Insert

   Lamp1_mStayGold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-2XPRE-GFP

   Plasmid

   #206169

   Purpose

   pMVP Gateway destination vector (3rd generation lentiviral vector) containing 2 consensus PGR binding sites upstream of a minimal 2YBTATA promoter

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   2X PRE-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   2YBTATA

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPHAGE_DLD-H450A-C-TAP

   Plasmid

   #83478

   Purpose

   Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   H450A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHAGE_DLD-S456A-C-TAP

   Plasmid

   #83479

   Purpose

   Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

   Depositor

   Mohan Babu

   Article

   Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

   Insert

   DLD (DLD Human)
   

   Use

   Lentiviral

   Tags

   TAP

   Expression

   Mammalian

   Mutation

   S456A

   Promoter

   CMV

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

   Plasmid

   #199483

   Purpose

   Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

   Depositor

   Robert Bryson-Richardson

   Article

   Miles et al bioRxiv 2023

   Insert

   Gal4vp16/4xnrUAS-mKate2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPS0382

   Plasmid

   #133232

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRJPaph-sYFP2

   Plasmid

   #67033

   Purpose

   Plasmid for Paph-sYFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

   Depositor

   Julia Vorholt

   Article

   Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

   Inserts

   B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
   sYFP2
   

   Use

   Conjugative bacterial vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBindCY

    Plasmid

    #158223

    Purpose

    Expressing CheZ protein in response to blue light

    Depositor

    Chueh Loo Poh

    Article

    Zhang et al J Mol Biol. 2020 May 1;432(10):3137-3148

    Insert

    CheZ
    

    Use

    Synthetic Biology

    Tags

    YbaQ

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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