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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pY007 (pcDNA3.1-hPeCpf1)

Plasmid

#69979

Purpose

Expresses humanized PeCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPeCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-RING1_1

Plasmid

#36355

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
2xFKBP-myc

Plasmid

#20212

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pTRIPZ-DoxOn-shCUX1-5150

Plasmid

#90469

Purpose

Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

Depositor

Alain Nepveu

Article

Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

Insert

TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.RCT

Plasmid

#184661

Purpose

Retroviral expression of mouse RAP1 delta RCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta RCT

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIC_P2A_Hygro_Barcode

Plasmid

#120484

Purpose

Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIC (SPIC Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Vh-HA-2xFKBP

Plasmid

#20221

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Aps-SH2

Plasmid

#46404

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Aps (SH2B2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 404-523

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Myc-CD4-mCherry-HA-FRB

Plasmid

#100740

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD4

Tags

FRB, HA, Myc, and mCherry

Expression

Mammalian

Mutation

Engineered to remove large part of extracellular …

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag Jnk1a1(APY)

Plasmid

#15742

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1(TPF) (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Mutation

Thr-190Ala

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
BirA_BVES untagged

Plasmid

#196581

Purpose

expresses BirA

Depositor

Cheryl Arrowsmith

Article

Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

Insert

BirA_Ecoli

Use

Baculovirus expression

Available Since

June 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSMP-MBD2_2

Plasmid

#36369

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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