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pAS4.1w-tet-on-NORAD
Plasmid#125010Purposeexpress in mammalian cellDepositorInsertHomo sapiens non-coding RNA activated by DNA damage (NORAD), long non-coding RNA (NORAD Human)
UseTagsExpressionMammalianMutationPromoterTRE-TightAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-A120E
Plasmid#55687Purposeexpresses constitutively active human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120EPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pJ414-PCY1:S495A
Plasmid#98151PurposeExpresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:S495A
UseTagsHis6-TEVExpressionBacterialMutationChanged serine 495 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:W603A
Plasmid#98153PurposeExpresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:W603A
UseTagsHis6-TEVExpressionBacterialMutationChanged tryptophan 603 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695Q
Plasmid#98154PurposeExpresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695Q
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to glutaminePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:H695A
Plasmid#98155PurposeExpresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:H695A
UseTagsHis6-TEVExpressionBacterialMutationChanged histidine 695 to alaninePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:Y696G
Plasmid#98156PurposeExpresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:Y696G
UseTagsHis6-TEVExpressionBacterialMutationChanged tyrosine 696 to glycinePromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2G2A
Plasmid#174334Purposeencodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationmutation of a.a 2 Gly to AlaPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FG11F-C-FLAG-hNKD2
Plasmid#174330Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseLentiviralTagsFLAG TagExpressionMammalianMutationPromoterUbCAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P1
Plasmid#122645PurposeExpresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P3
Plasmid#122647PurposeExpresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P4
Plasmid#122648PurposeExpresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
UseTagsHis-tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGS1122
Plasmid#63790PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection markerDepositorInserteSapphire-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseSapphire-Twin-Strep tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionTagsExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 WT
Plasmid#12679DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 WT
Plasmid#12661DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab11 WT
Plasmid#12674DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab11 DN
Plasmid#12678DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsGFP and HAExpressionMammalianMutationchanged serine 25 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP 695
Plasmid#30137DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695PromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-695)/pcDNA3.1
Plasmid#111453PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTags8xHis and EGFPExpressionMammalianMutationKv7.4 (1-695)PromoterAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 DN
Plasmid#12680DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationchanged serine 25 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab7 DN
Plasmid#12662DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsDsRed and HAExpressionMammalianMutationT22NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_003
Plasmid#192505PurposeFor cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFPDepositorInserthU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab7 WT
Plasmid#12605DepositorInsertRAB7 (Homo sapiens) (RAB7A Human)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAX FLAG APP
Plasmid#30154DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsFLAGExpressionMammalianMutationwild type splice variant 751PromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Kv7.1/pcDNA3.1
Plasmid#111452PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
UseTags8xHis and EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0517
Plasmid#141627PurposeLentiviral vector for overexpressing the PAX5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX5 (PAX5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAX APP 751
Plasmid#30138DepositorInsertAmyloid Precursor Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 751PromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pCAX APP C99
Plasmid#30146DepositorInsertAPP C99 (APP Human)
UseTagsExpressionMammalianMutationC-terminal from BACE site of APPPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_AJMA
Plasmid#183760PurposeBxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703DepositorInsertsASAP2f
jRCaMP1b
miRFP703
ASAP2f
UseTagsLck and NESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-Nanog-Pur
Plasmid#16578DepositorInsertHomo sapiens Nanog homeobox (NANOG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-n1-AICD
Plasmid#69925Purposemammalian expression of human AICD fused to EGFPDepositorInsertAICD (APP Human)
UseTagsEGFPExpressionMammalianMutation47 aa longPromoterCMVAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only
