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Showing: 521 - 540 of 62682 results

  2. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Plasmids to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system (Addgene #1000000080).
    Depositor
    Mikhail Shapiro
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  6. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Article
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

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  7. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Glu

    Plasmid
    #218458
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Glu substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Thr1273Glu substitution
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218458-plasmid-map-sequence-id-466904

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  8. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-His1271Lys

    Plasmid
    #218456
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (His1271Lys substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    His1271Lys substitution
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218456-plasmid-map-sequence-id-466903

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  9. BD-pGEX5X3-SARS-CoV-2-S-tail-Lys1269Ala-His1271Ala

    Plasmid
    #218459
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Lys1269Ala and His1271Ala substitutions) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag (S SARS-CoV-2)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Lys1269Ala and His1271Ala substitutions
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218459-plasmid-map-sequence-id-466902

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  11. JO-pH720-SARS-CoV-2-S-tail-Cys1254Ala-Thr1273Glu

    Plasmid
    #218454
    Purpose
    Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike (with Cys1254Ala and Thr1273Glu substitutions) and an N-terminal eXact tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag and Cys1254Ala and Thr1273Glu substitutions (S SARS-CoV-2)
    Tags
    eXact
    Expression
    Bacterial
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218454-plasmid-map-sequence-id-436194

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  12. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp

    Plasmid
    #218457
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Thr1273Asp substitution
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218457-plasmid-map-sequence-id-436203

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  16. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available Since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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  20. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

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Showing: 521 - 540 of 62682 results