We narrowed to 5,035 results for: mcs

1. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

   Plasmid

   #199346

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. V309 - pDNR MCS SA lacZ alpha - Donor

   Plasmid

   #11692

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Donor vector

   Available Since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)

   Plasmid

   #105922

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITR

   Depositor

   Marcella Patrick

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available Since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pSB-CMV-MCS-Puro GEM-LTB4

   Plasmid

   #202641

   Purpose

   Expresses the LTB4 sensor GEM-LTB4 in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSB-CMV-MCS-Puro GEM-LTB4mut

   Plasmid

   #202642

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4mut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p5E-MCS-c-Fos-b-globin (JDW 1237)

   Plasmid

   #229829

   Purpose

   A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   c-fos minimal promoter and b-globin intron
   

   Use

   Gateway entry clone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Syn-FRT-MCS-WPRE-SV40 - GCH9

   Plasmid

   #230819

   Purpose

   AAV cloning vector for Flpo-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV; Cloning vector

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2T-CAG-MCS-P2A-GFP-PuroR

    Plasmid

    #107186

    Purpose

    Base plasmid for cloning gene duplication alleles with eGFP reporter.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Type

    Empty backbone

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJT040 lenti MCS pEF mIFP WPRE

    Plasmid

    #161929

    Purpose

    Lentiviral expression of mIFP with multiple cloning site upstream the pEF promoter

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    mIFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. V308 pDNR MCS lacZ alpha - Donor

    Plasmid

    #11691

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

    Plasmid

    #199347

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-3C

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

    Plasmid

    #139788

    Purpose

    Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

    Depositor

    Arneet Saltzman

    Article

    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

    Type

    Empty backbone

    Tags

    6xHis (cleavable by TEV protease) and GST (cleava…

    Expression

    Bacterial

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

    Plasmid

    #166413

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    Protection Of Telomeres 1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB deleted 125 a.a

    Promoter

    ef1-a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

    Plasmid

    #199357

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

    Plasmid

    #64314

    Purpose

    chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

    Depositor

    Noriyuki Tsumaki

    Article

    Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

    Inserts

    COL11A2 promoter (COL11A2 Human)
    COL11A2 enhancer (COL11A2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and IRES-PURO

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

    Plasmid

    #62931

    Purpose

    pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    1.3αCaMKII

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

    Plasmid

    #69903

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge