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pCART0017_CROPseq-CAR-Puro_GD2-BBz

Plasmid

#242985

Purpose

GD2-BBz 14g2a CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GD2-BBz 14g2a CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MCL1

Plasmid

#202663

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-CAR

Plasmid

#157743

Purpose

knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ133-tRNA

Plasmid

#179212

Purpose

Golden Gate entry vector; 3rd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Promoter

no promoter

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1

Plasmid

#166964

Purpose

Expresses Myc-tagged KLC1 protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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p32Lb

Plasmid

#158154

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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61426-hU6-shmFmr1-EF1a-mCherry

Plasmid

#157859

Purpose

Expresses a shRNA targeting mouse Fmr1 gene

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Fmr1 (Fmr1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-NSP16

Plasmid

#154414

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-NSP14

Plasmid

#154412

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

Plasmid

#132409

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRanCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H147A; H1044A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-Nono-sh1

Plasmid

#127650

Purpose

Knock-down of human NONO

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

Insert

NONO shRNA (NONO Human)

Use

Lentiviral

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 ERG R367K

Plasmid

#118626

Purpose

Overexpresses ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG ( R367K DNA Binding mutant) (ERG Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

R367K

Promoter

CMV

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

Plasmid

#112214

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)

Use

CRISPR

Tags

mCherry

Mutation

D10A,H840A,N863A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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AE-Polyubiquitin-GCaMP6s

Plasmid

#106868

Purpose

Expresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.

Depositor

Omar Akbari

Article

Bui et al BMC Neurosci. 2019 Jun 17;20(1):27. doi:

Insert

AE-Polyubiquitin-GCAaMP6s

Expression

Insect

Promoter

Ae. aegypti polyubiquitin (PUb)

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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shBCL2L1 # 1

Plasmid

#42551

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBCL2L1 (BCL2L1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
shBCL2L1 # 2

Plasmid

#42552

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shBCL2L1 (BCL2L1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
shTCF7L2 # 1

Plasmid

#42557

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTCF7L2 (TCF7L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pSLQ10622

Plasmid

#239274

Purpose

Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ancBE4-NG

Plasmid

#226853

Purpose

Expresses ancBE4max-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ancBE4max-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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