Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 11,563 results for: Vars;

Showing: 5381 - 5400 of 11563 results

  2. NanoLuc-PM

    Plasmid
    #164784
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    Use
    Tags
    NanoLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164784-plasmid-map-sequence-id-326673

    Enlarge

  7. pCW57.1-FOXM1b

    Plasmid
    #68811
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1b (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68811-plasmid-map-sequence-id-419548

    Enlarge

  9. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

    Enlarge

  13. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

    Enlarge

  17. pCW57.1-FOXM1c

    Plasmid
    #68810
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1c (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68810-plasmid-map-sequence-id-232051

    Enlarge

  18. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

    Enlarge

  19. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

    Enlarge

  20. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

    Enlarge

Showing: 5381 - 5400 of 11563 results