We narrowed to 36,681 results for: KAN

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pDONR221_SLC44A2

Plasmid

#132214

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC44A2 (SLC44A2 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLCO1B1

Plasmid

#132188

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B1 (SLCO1B1 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_L1-YFP-miniTurbo-NES-L2

Plasmid

#127351

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

Plasmid

#130816

Purpose

Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bJun-NFAST-NLS-DEVDG-mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-3xFLAG-G

Plasmid

#127914

Purpose

a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Tags

mNeonGreen-3xFlag

Expression

Mammalian

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EpsilonCOP EGFP

Plasmid

#128151

Purpose

Expression of epsilon COP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

epsilon COP (COPE Bovine)

Expression

Mammalian

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoTGFBRs

Plasmid

#118942

Purpose

Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

Depositor

Won Do Heo

Article

Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

Insert

OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pIRESh-GFPII-MB-Dlk1

Plasmid

#108930

Purpose

GFP-tagged vector expressing membrane-bound Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Mutation

lacks coding sequence for the juxtamembrane motif…

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-AF8

Plasmid

#117803

Purpose

mammalian expression of GFP-tagged human centromere protein A

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2000 Aug;25(4):253-61

Insert

CENP-A (CENPA Human)

Tags

GFP

Expression

Mammalian

Mutation

See depositor comments below.

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2L348F(1-531)-mCh-BAXS184E

Plasmid

#117239

Purpose

Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

Cry2531.L348F-mCh-BAXS184E

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAM4891 (pCV0003)

Plasmid

#120080

Purpose

Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2

Use

Other

Mutation

mobA Y25F

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE135

Plasmid

#118489

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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EB3-3xmScarletI-N1

Plasmid

#112928

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mScarletI_MapTauC

Plasmid

#112964

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

MapTauC

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pmIL-6promoterEGFP

Plasmid

#112896

Purpose

To monitor the activation of the murine IL-6 promoter by eGFP expression

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Promoter

IL-6 promoter

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pKISox1-P2A-eGFP-Hygro

Plasmid

#115685

Purpose

Knock-in of P2A-eGFP at the mouse Sox1 gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-eGFP-loxP-PGK-Hygro-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Human HUWE1 (3993-4374)

Plasmid

#110754

Purpose

Bacterial Expression of HUWE1 HECT domain

Depositor

David Komander

Article

Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

Insert

HUWE1 (HUWE1 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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