We narrowed to 5,924 results for: pcDNA 3.1

1. CIB-VP16 (B1014)

   Plasmid

   #92036

   Purpose

   Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Fusion of plant CIB1 (intact NLS) with VP16 activ…

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLYLQ

   Plasmid

   #80421

   Purpose

   expresses cDNA for human CELF1 (CUGBP1) Flag tagged

   Depositor

   Thomas Cooper

   Article

   Kalsotra et al Proc Natl Acad Sci U S A. 2008 Dec 23;10

   Insert

   human CELF1 cDNA (CELF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Opto-GPR4

   Plasmid

   #106001

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR4 (GPR4 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Arrb1-Q394L

   Plasmid

   #35402

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q394L

   Promoter

   CMV

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Nuc-mTurq2-Apollo-NADP+

   Plasmid

   #193302

   Purpose

   Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

   Depositor

   Jonathan Rocheleau

   Article

   Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

   Insert

   G6PD (G6PD Human)
   

   Use

   Tags

   SV40 NLS and mTurqoise2

   Expression

   Mammalian

   Mutation

   S40A, R72Q, H201N, K205T

   Promoter

   T7

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGATA-1 K137R

   Plasmid

   #118353

   Purpose

   This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K137R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald(luminal)-canxTM

   Plasmid

   #186961

   Purpose

   Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CANX (CANX Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Only express calnexin (482-end) with ER signal pe…

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB07 (CK2beta)

   Plasmid

   #27091

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. NSP14-Flag

    Plasmid

    #210346

    Purpose

    Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3s-ABE7.10

    Plasmid

    #113128

    Purpose

    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE 7.10
    

    Use

    Tags

    HA epitope and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Opto-GPR3

    Plasmid

    #106000

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR3 (GPR3 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyCas9-AIDx

    Plasmid

    #135343

    Purpose

    Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. bArr1-ALFA

    Plasmid

    #201496

    Purpose

    Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr1-ALFA (ARRB1 Human)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

19. mH2A2-CT-MYC

    Plasmid

    #45170

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy2 (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. ABEMax nSaCas9

    Plasmid

    #135364

    Purpose

    Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax nSaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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