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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,106 results for: SON

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Showing: 5421 - 5440 of 31106 results

  1. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  8. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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  9. PhyB-mCherry-Tom7

    Plasmid
    #66571
    Purpose
    Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae
    Depositor
    Orion Weiner
    Article
    Jost et al ACS Synth Biol. 2015 Jun 2.
    Insert
    PhyB (PHYB Mustard Weed)
    Tags
    Tom7 and mCherry
    Expression
    Yeast
    Mutation
    PhyB portion contains aa's 1-908
    Promoter
    pADH1
    Available Since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66571-plasmid-map-sequence-id-126395

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  10. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Expression
    Bacterial
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  11. mPA-GFP-MAPTau-N-10

    Plasmid
    #57146
    Purpose
    Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    MAPTau (MAPT Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57146-plasmid-map-sequence-id-263558

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  15. CoV2-Spike-D614G-Alpha

    Plasmid
    #177961
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)
    Depositor
    Melanie Ott
    Article
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Insert
    SARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
    Expression
    Mammalian
    Mutation
    del69, 70, del144, D614G, N501Y, A570D, P681H, T7…
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177961-plasmid-map-sequence-id-348264

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  19. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160140-plasmid-map-sequence-id-317331

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Showing: 5421 - 5440 of 31106 results