We narrowed to 9,180 results for: mel

1. pET28b-NmCas9-Flag D16A+H588A

   Plasmid

   #109033

   Purpose

   D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-NmCas9-Flag H588A

   Plasmid

   #109032

   Purpose

   H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-R1ACR_741-EYFP

   Plasmid

   #103961

   Purpose

   Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   Anion channelrhodopsin R1ACR_741
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDsgRNA-pfkB

   Plasmid

   #102284

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   pfkB gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKDsgRNA-xylA

   Plasmid

   #89964

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   xylA gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF1-ERBB4 (R544W)

   Plasmid

   #29540

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCS2+8-aex-5-flag-tev-halo-his

   Plasmid

   #227152

   Purpose

   For T-REX experiments of aex-5.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   aex-5 (aex-5 Nematode)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+8-asp-2-flag-tev-halo-his

   Plasmid

   #227153

   Purpose

   For T-REX experiments of asp-2.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   asp-2 (asp-2 Nematode)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221818

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT7R homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

    Plasmid

    #221819

    Purpose

    Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    GFP11-HA donor with homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221832

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT1AR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221833

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2BR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

    Plasmid

    #221835

    Purpose

    Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    2xFLAG with dSERT homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221837

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT1BR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221838

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221839

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPOTv7-hyg-OsAID (p5110)

    Plasmid

    #239360

    Purpose

    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Inserts

    Rice Auxin Inducible Degradation domain
    Auxin Inducible Degradation domain
    

    Use

    Bacterial

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPOTv6-puro-3Ty:OsAID:3Ty (p5229)

    Plasmid

    #239361

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    none

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)

    Plasmid

    #239362

    Purpose

    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin

    Depositor

    Mark Carrington

    Article

    Alexandra et al bioRxiv 2025.06.17.660065

    Insert

    Rice Auxin inducible degradation domain
    

    Use

    Bacterial

    Tags

    3 x Ty epitope tag

    Promoter

    n/a

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge