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We narrowed to 21,298 results for: plasmids 101

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Showing: 5461 - 5480 of 21298 results

  1. pIS1 mHK1

    Plasmid
    #60795
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains HK1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    HK1 3'UTR and mutated miR-155 binding site (HK1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60795-plasmid-map-sequence-id-97806

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  2. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  3. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

    Plasmid
    #193356
    Purpose
    VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2-17 is engineered highly cooperative chimeric…
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193356-plasmid-map-sequence-id-379848

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  4. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  5. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  6. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  7. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  8. pGL3Basic-ME.2/ApoEpromoter

    Plasmid
    #51436
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer
    Depositor
    Sohail Tavazoie
    Article
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Insert
    APOE apolipoprotein E (APOE, ME2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    The ME.2 enhancer is fused upstream of the ApoE g…
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51436-plasmid-map-sequence-id-177530

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  9. pGL3Basic-ME.2/ApoEpromoter

    Plasmid
    #51436
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer
    Depositor
    Sohail Tavazoie
    Article
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Insert
    APOE apolipoprotein E (APOE, ME2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    The ME.2 enhancer is fused upstream of the ApoE g…
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51436-plasmid-map-sequence-id-177530

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  18. pIS1 mEPAS1

    Plasmid
    #60793
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60793-plasmid-map-sequence-id-112560

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  19. pIS1 mEPAS1

    Plasmid
    #60793
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60793-plasmid-map-sequence-id-112560

    Enlarge

Showing: 5461 - 5480 of 21298 results