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pECE-M2-PPP1R12C wt

Plasmid

#31659

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)

Tags

M2

Expression

Mammalian

Mutation

codon-optimized

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
Krox20-MSE8 pGL3-TATA

Plasmid

#21261

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

Krox20-Myelin Specific Enhancer (Egr2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 MDM2 S186D

Plasmid

#16235

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
OMM-V5-LOV-Turbo

Plasmid

#199665

Purpose

expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

AKAP1 transmembrane domain and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 ORF3A_nostop

Plasmid

#149319

Purpose

Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR207 SARS-CoV-2 NSP1_nostop

Plasmid

#149304

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Dynamin I-K44A-non-tagged

Plasmid

#32695

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
996 pSG5L HA PTEN S380A

Plasmid

#10755

Depositor

William Sellers

Article

Vazquez et al Mol Cell Biol. 2000 Jul . 20(14):5010-8.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

S380A

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
N370S GBA pcDNA3.1

Plasmid

#188583

Purpose

Ectopic expression N370S GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

N370S

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-Neo/Myc PD-L1

Plasmid

#121477

Purpose

expression of human Myc-PD-L1 in mamalian cell

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

Myc

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His WT CRres si2,4R

Plasmid

#139404

Purpose

Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Promoter

pCMV, TRex

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-proα1(I)

Plasmid

#119843

Purpose

Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α1 chain (Col1a1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a1 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKG139

Plasmid

#69755

Purpose

Retroviral expression of CENP-C

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-C (CENPC Human)

Use

Retroviral

Tags

mCherry-TEV-S

Expression

Mammalian

Mutation

L341F, N570D relative to NP_001803.2 (numbering b…

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_TNF-SBP-mCherry

Plasmid

#65283

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NQO1

Plasmid

#61730

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Adamovich et al Mol Cell Biol. 2013 Jul;33(13):2603-13.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCold-TF-hPLEKHM1

Plasmid

#64146

Purpose

For protein expression in E.coli

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Tags

6xHis-tag and Trigger Factor (TF)

Expression

Bacterial

Promoter

cspA

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-flag-mKdm4d(H189A)-polyA

Plasmid

#61554

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (catalytic mutant)

Tags

flag

Expression

Mammalian

Mutation

changed Histidine 189 (of Kdm4d) to Alanine

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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