We narrowed to 9,277 results for: Pol;

1. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-HA_Ubiquitin

   Plasmid

   #131258

   Purpose

   Mammalian expression of ubiquitin with an N-terminal HA tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin C (UBC Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV enhancer + CMV promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SYN-H-sDarken

   Plasmid

   #184797

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetO-FUW-PBRM1-pgk-puro

   Plasmid

   #85746

   Purpose

   tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

   Depositor

   Emily Dykhuizen

   Article

   Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   none

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQHA-USP11 CS puroR

   Plasmid

   #46750

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   p10 and polyhedrin

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. W118-1_hTGM3-Flag

   Plasmid

   #180408

   Purpose

   lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM3 (TGM3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 Human 3' HP1a gRNA

   Plasmid

   #127907

   Purpose

   WT Cas9 Vector targeting the 3' end of the human HP1a gene

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   gRNA for Human 3' HP1a
   

   Use

   CRISPR

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-NLS-Cre-2A-mCherry-FKF

   Plasmid

   #119868

   Purpose

   Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-OT (wt TCF-4 sites)

    Plasmid

    #16558

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    TCF-4 binding sites (TCF4 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    3 copies of wildtype Tcf-4 binding sites

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_WT RAD23B

    Plasmid

    #201439

    Purpose

    Expresses human RAD23B with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SYN-L-sDarken

    Plasmid

    #184798

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. W118-1_hTGM4-Flag

    Plasmid

    #180409

    Purpose

    lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM4 (TGM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. W118-1_hTGM5-V5

    Plasmid

    #180414

    Purpose

    lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM5 (TGM5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDT386

    Plasmid

    #174733

    Purpose

    Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    T5

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T3-GST-EGFP

    Plasmid

    #177136

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    EGFP
    

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-puro-mycRSK2wt

    Plasmid

    #15827

    Depositor

    Jing Chen

    Article

    Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

    Insert

    Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    Nov. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-Tagger

    Plasmid

    #129396

    Purpose

    Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

    Insert

    rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
    

    Tags

    FLAG, HA, and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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