We narrowed to 9,277 results for: Pol;

1. pVETL-Cmcs-Flag-Ataxin3Q80-C14A

   Plasmid

   #22121

   Depositor

   Henry Paulson

   Article

   Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C14A

   Available Since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5FRT/TO-UBE2QL1-Strep/HA

   Plasmid

   #124666

   Purpose

   Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS1988

   Plasmid

   #49119

   Purpose

   AAV plasmid with CAGGS promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX Flag RagC T90N

   Plasmid

   #112758

   Purpose

   express Flag-RagC (T90N mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (T90N) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   T90N

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLyGo-Ec-7

   Plasmid

   #163135

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Nanobody, C-IgAP autotransporter

   Expression

   Bacterial

   Promoter

   P(rhaB)

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-N-mEGFP-PARP6

   Plasmid

   #178005

   Purpose

   Expresses N-terminally mEGFP-tagged PARP6

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP6 (PARP6 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaV1.3e[8a,11,31b,Δ32,42a] mut

   Plasmid

   #26576

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, 31b and 42a.Deletion of ex…

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

   Plasmid

   #129513

   Purpose

   Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 C88S (UBE2QL1 Human)
   

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-GGG-HIS-CBM-Xmod-DocIII

   Plasmid

   #91698

   Purpose

   CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-HIS-CBM-Xmod-DocIII
   

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNCX1 HA Akt1

    Plasmid

    #15990

    Depositor

    Morris Birnbaum

    Article

    Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 GFP RagB Q99L

    Plasmid

    #112749

    Purpose

    express GFP-RagB (Q99L mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    GFP-RagB (Q99L) (RRAGB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Q99L

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_Sik3_T142Q

    Plasmid

    #119973

    Purpose

    Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Sik3 (Sik3 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    change Threonine 142 to Glutamine

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6P1-Ataxin3Q22

    Plasmid

    #22117

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-dCas13b-ADAR2DD

    Plasmid

    #190913

    Purpose

    Addgene #103871 with 3x FLAG on N-terminal of dCas13

    Depositor

    Timothy Jarome

    Article

    Farrell et al Mol Psychiatry. 2023 May 17. doi: 10.103

    Insert

    3X FLAG
    

    Use

    CRISPR

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDF11-SFPQ(276-598)

    Plasmid

    #135437

    Purpose

    Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Splicing Factor Proline And Glutamine Rich (SFPQ Human)
    

    Tags

    hexahistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCellFree_G03 BATF3

    Plasmid

    #67050

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BATF3 (BATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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