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pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSP568

Plasmid

#139493

Purpose

Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xMS2sl

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
pIVEX2.3-Theo-3c-EGFP

Plasmid

#217526

Purpose

Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

Theo-3c riboswitch-regulated EGFP

Use

In vitro transcription-translation

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-p65-HSF1-Blast

Plasmid

#192652

Purpose

3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-p65-HSF1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZCm

Plasmid

#46764

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZKm

Plasmid

#46767

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_T2

Plasmid

#184864

Purpose

pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_T1

Plasmid

#184863

Purpose

pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry

Plasmid

#229146

Purpose

Promoter strength evaluation of PtlpA

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry

Expression

Bacterial

Mutation

No

Promoter

PtlpA

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAd5-B7-deltaE4

Plasmid

#122560

Purpose

Modified block 7, with deletion in E4

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 30888-35938

Use

Synthetic Biology

Mutation

Adenovirus sequences 32874-35461 replaced with Sp…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-PACT

Plasmid

#15667

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

PACT (PRKRA Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
Halo-MDC1

Plasmid

#207108

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMSSM24: pCMV-SpuFz1

Plasmid

#205260

Purpose

Human expression vector for human codon optimized SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Ca-FLARE (protease)

Plasmid

#92214

Purpose

protease component of FLARE

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

CaM-V5-TEVp(Δ220-242)

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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