We narrowed to 63,044 results for: cloning

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pDONR223-CARD11

Plasmid

#23482

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CARD11 (CARD11 Human)

Use

Gateway Cloning

Mutation

A291C,C292T,1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP3K5

Plasmid

#23517

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K5 (MAP3K5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KA3

Plasmid

#23527

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA3 (RPS6KA3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKCG

Plasmid

#23640

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCG (PRKCG Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAG2

Plasmid

#23689

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG2 (PRKAG2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-CDK4

Plasmid

#23778

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CDK4 (CDK4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pJA257

Plasmid

#21510

Depositor

Julie Ahringer

Publication

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR

Use

Gateway Cloning

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-LUC (w468-1)

Plasmid

#19135

Purpose

Gateway entry vector, V5-Luciferase

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Gateway Cloning

Tags

V5

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF-ENTR B (708-1)

Plasmid

#17428

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame B

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pnlacZ-ACN

Plasmid

#20304

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

nlacZ-ACN

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV-PHP.eB-CMV-eGFP

Plasmid

#230929

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Publication

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HDAC2

Plasmid

#222553

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HDAC2 (HDAC2 Human)

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Publication

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRT006.3 L1 dual-luciferase AI retrotransposition reporter

Plasmid

#213031

Purpose

Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

Depositor

Donna Romero, Martin S. Taylor

Publication

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker

Use

Luciferase

Tags

Firefly luciferase antisense intron (3' UTR)

Expression

Mammalian

Promoter

pTRE bidirectional tet-on

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-TMEM192-mKeima

Plasmid

#215358

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Publication

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mClover3-TDP-43ΔNLS

Plasmid

#214917

Purpose

inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

Depositor

Ophir Shalem

Publication

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

TDP-43ΔNLS (TARDBP Human)

Use

Lentiviral

Tags

mClover3

Mutation

mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Publication

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Publication

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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