We narrowed to 3,338 results for: UBC

1. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

   Plasmid

   #89793

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Q147L, CAA-->CTA

   Promoter

   hUbC

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

   Plasmid

   #216475

   Purpose

   lentiviral dual expression of YAP5SA and eGFP

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti-UBC- IKZF4-3xHA-pGK-PUR

   Plasmid

   #107384

   Purpose

   Lentiviral expression of IKZF4-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF4 (IKZF4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla

   Plasmid

   #174620

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter

   Depositor

   Volker Busskamp

   Article

   Gasparini et al J Clin Invest. 2022 Apr 28. pii: 154619.

   Insert

   enhanced green fluorescent protein
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

   Plasmid

   #203797

   Purpose

   Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Article

   Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

   Inserts

   PIK3CA (PIK3CA Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-UBC-DSRED-LUC-EF1 Hygro

   Plasmid

   #129437

   Purpose

   express dsred-luc chimera

   Depositor

   Oskar Laur

   Insert

   dsred-luc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

   Plasmid

   #225943

   Purpose

   Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Evan Reid, Richard Eva, Bart Nieuwenhuis

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #162334

    Purpose

    Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSaCas9-KRAB
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUBC-Pfv-Sapphire-IRES-H2B-mCherry

    Plasmid

    #203648

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire-IRES-H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

    Plasmid

    #106249

    Purpose

    Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB T2A PuroR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA-NLS and NLS-KRAB

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    hUbC

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

    Plasmid

    #214994

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

    Plasmid

    #83925

    Purpose

    Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    eGFP
    Bsr
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A

    Expression

    Mutation

    Promoter

    hUbC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid

    #158162

    Purpose

    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    2xV5-TurboID-NES
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    UBC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid

    #158160

    Purpose

    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-3xHA-miniTurbo-3XNLS
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

    Plasmid

    #164044

    Purpose

    Expression of stdMCP-tdTomato in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    stdMCP-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. plenti-UBC-Myc-gate-pGK-HYG

    Plasmid

    #107395

    Purpose

    Lentiviral gateway destination vector

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-UBC-Qa1 SCT-T2A-GFP

    Plasmid

    #205453

    Purpose

    lentiviral plasmid for expression of mouse Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Qa1 SCT (H2-T23 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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