167,318 results

1. AAV-Syn-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #125693

   Purpose

   Global labeling of excitatory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV14_Fibrillarin

   Plasmid

   #73061

   Purpose

   expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPgiCas13b

   Plasmid

   #184833

   Purpose

   Inducible expression of PgiCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Porphyromonas gingivalis
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. actc1b-mKate2-P2A-APEX2-VHH[GFP]

   Plasmid

   #126749

   Purpose

   Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   VHH[EGFP]
   

   Use

   Zebrafish muscle expression clone

   Tags

   mKate2-P2A-APEX2

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC25A31

   Plasmid

   #131969

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A31 (SLC25A31 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. mEmerald-FilaminA-N-9

   Plasmid

   #54098

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   FilaminA (FLNA Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pYY1704

   Plasmid

   #198421

   Purpose

   pBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.

   Depositor

   Yuan You

   Article

   You et al Nat Plants. 2023 Jan;9(1):128-141. doi:

   Insert

   RedNTF
   

   Expression

   Plant

   Promoter

   pBs3

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC25A51

    Plasmid

    #132019

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A51 (SLC25A51 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. makeTCR_Lentiviral_Backbone

    Plasmid

    #233943

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a (long)

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTopo_Ex2Donor_pPGK-PURO

    Plasmid

    #198862

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT Ex2

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    PURO
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. DH10B-M2.NmeMC58II

    Bacterial Strain

    #114268

    Purpose

    The strain expresses M2.NmeMC58II methylase and zeocin selection marker from the arsB locus.

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Bacterial Resistance

    Bleocin (zeocin)

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pBLxG2

    Plasmid

    #149472

    Purpose

    Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LuxR-GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLuxB

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG1.1

    Plasmid

    #173685

    Purpose

    The expression vector for genes of interest under CAG promoter.

    Depositor

    Masahito Ikawa

    Article

    Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR- flag-Lag16-DLL1

    Plasmid

    #205201

    Purpose

    This vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM DLL1 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-HA-Chk2-D311V

    Plasmid

    #41921

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D311V

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pOSV806

    Plasmid

    #126606

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EML4-ALK variant 1

    Plasmid

    #183828

    Purpose

    Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-13 and ALK exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA3-PAX3-FOXO1/FKHR

    Plasmid

    #115526

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

    Insert

    Pax3-FOXO1/FKHR (FOXO1, Pax3 Human, Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDONR221_SLC25A10

    Plasmid

    #132014

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A10 (SLC25A10 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. pxpr_050-wt-ltr

    Plasmid

    #182646

    Purpose

    lentiviral expression of gRNA scaffold, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKCyR6

    Plasmid

    #149493

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CymR(AM)-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PCymRC

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMRX-IP TFEB-sfGFP

    Plasmid

    #135402

    Purpose

    Expresses TFEB-sfGFP

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    TFEB (TFEB Human)
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pHAGE2-TetOminiCMV-SV40LT

    Plasmid

    #136616

    Purpose

    Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    SV40 large T antigen
    

    Use

    Lentiviral

    Expression

    Bacterial

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. 3541 pMIG Bcl-XL

    Plasmid

    #8790

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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30. pBItetDT960GFP

    Plasmid

    #80419

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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