We narrowed to 5,924 results for: pcDNA 3.1

1. hHSF1 S303A

   Plasmid

   #118363

   Purpose

   This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4999

   Plasmid

   #107555

   Purpose

   Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(dead1)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   D917A

   Promoter

   CMV

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MED11

   Plasmid

   #15418

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED11 (Med11 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. Opto-GPR37

   Plasmid

   #106019

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37; Opto-GPR37; B9)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR37 (GPR37 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CFP-GIV-CT FA

   Plasmid

   #69533

   Purpose

   Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

   Depositor

   Pradipta Ghosh

   Article

   Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

   Insert

   GIV (CCDC88A Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   C terminal amino acids S1660-S1870 of human GIV w…

   Promoter

   CMV

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-MED30

   Plasmid

   #15426

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED30 (Med30 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pJO427

   Plasmid

   #111139

   Purpose

   URT-type vector for Glu-mIκBα expression in Mammalian cells

   Depositor

   Alexander Varshavsky

   Article

   Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-MED20

   Plasmid

   #15421

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED20 (Med20 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. PA-RL-BAD

   Plasmid

   #78859

   Purpose

   DULIP positive control (bait) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 associated agonist of cell death (BAD Human)
   

   Use

   Expression vector

   Tags

   Protein A, Renilla luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTE4565

    Plasmid

    #88903

    Purpose

    Expresses inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CFP-GIV-CT WT

    Plasmid

    #69532

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV

    Promoter

    CMV

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-GPR182

    Plasmid

    #106061

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR182; Opto-GPR182; F3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR182 (GPR182 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-MED31

    Plasmid

    #15427

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED31 (Med31 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. Opto-GPR17

    Plasmid

    #106005

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR17; Opto-GPR17; A7)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR17 (GPR17 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJO428

    Plasmid

    #111141

    Purpose

    URT-type vector for Val-mIκBα expression in Mammalian cells

    Depositor

    Alexander Varshavsky

    Article

    Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Opto-GPR37L1

    Plasmid

    #106020

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR37L1 (GPR37L1 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE4567

    Plasmid

    #107557

    Purpose

    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hMbCpf1(dead2)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    E1080A

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Opto-GPR19

    Plasmid

    #106007

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR19 (GPR19 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTBL681 CHYRON4 integration construct

    Plasmid

    #126449

    Purpose

    To integrate the CHYRON4 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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