We narrowed to 19,248 results for: Tat

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pTH353-SUP45wt

Plasmid

#29371

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene including genomic sequence 1432 bp…

Available Since

May 5, 2011

Availability

Academic Institutions and Nonprofits only

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SGEP-sh-Hs-PHGDH-1883

Plasmid

#188669

Purpose

shRNA

Depositor

Lewis Cantley

Insert

shPHGDH (PHGDH Human)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTPN11-Q79R

Plasmid

#116646

Purpose

Lentiviral expression of PTPN11 Q79R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTPN11 (PTPN11 Human)

Use

Lentiviral

Mutation

Q79R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PTEN-R130G

Plasmid

#116630

Purpose

Lentiviral expression of PTEN R130G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R130G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316-GFP-COF1

Plasmid

#31843

Depositor

David Drubin

Article

Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

Insert

Cofilin (COF1 Budding Yeast)

Tags

GFP (S65T)

Expression

Yeast

Promoter

T7

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pET-Impact-T4gp43(exo-)

Plasmid

#228202

Purpose

Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A

Depositor

Stephen Benkovic

Insert

43

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Mutated aspartate 219 to alanine (D219A), elimina…

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Tags

9xMyc

Expression

Yeast

Promoter

pADH1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-gst-xr

Plasmid

#214761

Purpose

Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

Depositor

Pimchai Chaiyen

Article

Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

Insert

glutathione S-transferases fused with xylose reductase

Tags

Glutathione S-transferases

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 FTDP-17 N279K splicing minigene

Plasmid

#122967

Purpose

expresses circular tau RNAs with the FTDP-17 N279K

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

maps

Expression

Mammalian

Mutation

FTDP-17 mutation

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Expression

Mammalian

Promoter

Human U6

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-V344G

Plasmid

#116547

Purpose

Lentiviral expression of PIK3CA V344G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

V344G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-Q546R

Plasmid

#116530

Purpose

Lentiviral expression of PIK3CA Q546R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

Q546R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-NLGN4 D396*

Plasmid

#59321

Purpose

Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

Depositor

Peter Scheiffele

Article

Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

Insert

NLGN4 D396* (NLGN4X Human)

Tags

HA

Expression

Mammalian

Mutation

D396* nonsense mutation, rest of ORF deleted

Promoter

CAG

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway Cloning

Mutation

E179K

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
nonphosphorylatable Mis12-targeted Aurora B FRET sensor

Plasmid

#45229

Depositor

Michael Lampson

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

non phosphorylatable Mis12-targeted Aurora B FRET sensor

Expression

Mammalian

Mutation

T-A mutation in substrate sequence

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR)

Plasmid

#238123

Purpose

bacterial expression of human TTR

Depositor

Peter Wright

Article

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine

Promoter

T7

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKIF1C_R169W-GFP

Plasmid

#185981

Purpose

Mammalian expression of human KIF1C with R169W mutation and GFP tag.

Depositor

Anne Straube

Article

Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

Insert

KIF1C (KIF1C Human)

Tags

eGFP

Expression

Mammalian

Mutation

R169W - mutation causing HSP (SPG58)

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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