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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,463 results for: Pol;

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Showing: 8491 - 8520 of 9463 results

  3. pSLQ10871

    Plasmid
    #183962
    Purpose
    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG and U6
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183962-plasmid-map-sequence-id-363117

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  4. pSSA-RPG

    Plasmid
    #85932
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85932-plasmid-map-sequence-id-172472

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  6. pOCC305

    Plasmid
    #118898
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and TwinStrep-MB…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118898-plasmid-map-sequence-id-232364

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  9. ULK1-Halo HRD

    Plasmid
    #207548
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    207548-plasmid-map-sequence-id-430489

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  10. pFB-LIC-Bse

    Plasmid
    #26108
    Purpose
    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Opher Gileadi
    Article
    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His6 - TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Oct. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    26108-plasmid-map-sequence-id-12260

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  13. pTK331

    Plasmid
    #59572
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59572-plasmid-map-sequence-id-92977

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  18. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Article
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available Since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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  22. pYFP-NEAT1m_v1-RT

    Plasmid
    #97089
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1m_v1-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97089-plasmid-map-sequence-id-189689

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  24. pYFP-NEAT1_IS_RT

    Plasmid
    #97091
    Purpose
    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1_IS_RT (NEAT1 Human)
    Use
    CRISPR
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97091-plasmid-map-sequence-id-189691

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  29. pNHEJ-RPG

    Plasmid
    #85931
    Purpose
    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
    Depositor
    Zhiying Zhang
    Article
    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-
    Insert
    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    Use
    Dual-reporter surrogate
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85931-plasmid-map-sequence-id-172473

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Showing: 8491 - 8520 of 9463 results