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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,419 results for: 221

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Showing: 5661 - 5680 of 8419 results

  1. LPAR4-DuET

    Plasmid
    #213331
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR4 (LPAR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213331-plasmid-map-sequence-id-430865

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  2. HA-HA-AR-DBD

    Plasmid
    #171238
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171238-plasmid-map-sequence-id-336038

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  3. GPR120-DuET

    Plasmid
    #213261
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR120 (FFAR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213261-plasmid-map-sequence-id-430811

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  7. HA-AR-LBD

    Plasmid
    #171239
    Purpose
    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171239-plasmid-map-sequence-id-336014

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  9. pcDNA3HA-AR-LBD

    Plasmid
    #89111
    Purpose
    mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag
    Depositor
    Elizabeth Wilson
    Article
    He et al J Biol Chem. 1999 Dec 24;274(52):37219-2
    Insert
    hAR ligand binding domain (AR Human)
    Tags
    2xHA
    Expression
    Mammalian
    Mutation
    contains Ligand Binding Domain (624-919)
    Promoter
    CMV
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89111-plasmid-map-sequence-id-176143

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  11. LPAR4-Tango

    Plasmid
    #66420
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    LPA4 (LPAR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE

    Plasmid
    #166970
    Purpose
    Cre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.
    Depositor
    Matthew Banghart
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    Homo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
    Use
    AAV and Cre/Lox
    Tags
    FLAG, T2A, and mCherry
    Expression
    Mammalian
    Promoter
    hsyn
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166970-plasmid-map-sequence-id-346867

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Showing: 5661 - 5680 of 8419 results