We narrowed to 7,913 results for: RAP

Showing: 5701 - 5720 of 7913 results

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pGEMT-GDNF

Plasmid

#119897

Purpose

Mouse GDNF In situ hybridization probe

Depositor

Wenqin Luo

Article

Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

Insert

glial cell line derived neurotrophic factor (Gdnf Mouse)

Use

In situ hybridization probe

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMS4 (Empty insertion vector with SEC)

Plasmid

#154837

Purpose

Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

SEC

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

sqt-1(e1350)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A G120

Plasmid

#123071

Purpose

Gateway entry clone encoding human MAP1LC3A G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acid 121. Stop codon after G120.

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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ARB365

Plasmid

#124048

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis and AviTag

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMuLE ENTR SV40-hCas9 L5-L2

Plasmid

#62134

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

human codon optimized Cas9

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1788

Plasmid

#154317

Purpose

CT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1787

Plasmid

#154316

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV-hCas9 R4-R3

Plasmid

#62132

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

human codon optimized Cas9

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR418

Plasmid

#113875

Purpose

expression of double Cas9 programming sgRNA11 targetting STL1

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

doble sgRNA11-STL1

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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R0063 (pLuxR-pR)_AB

Plasmid

#66012

Purpose

MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120

Plasmid

#123074

Purpose

Gateway entry clone encoding human MAP1LC3B G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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myc-Med4 pBacPAK8

Plasmid

#15364

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

Med4 (MED4 N/A, Human)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagC(GD)/RagB(CTD) chimera

Plasmid

#112774

Purpose

express Flag-RagC (G domain) fused with with RagB (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB(GD)/RagC(CTD) chimera

Plasmid

#112773

Purpose

express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pX61

Plasmid

#46852

Purpose

TOP Hupki-puro2A-Hupki-wt plasmid

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 wild type (TP53 Human, Mouse)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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