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pET28_6xHis-PARP1delZn3

Plasmid

#173936

Purpose

Bacterial expression of PARP1 lacking Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1 D294A

Plasmid

#60542

Purpose

Donor vector for human MLK1 D294A mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Mutation

D294A

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pBS-AC3CtermGenomic-mCherry

Plasmid

#105250

Purpose

mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGPN-ACE2

Plasmid

#187483

Purpose

Expression vector - ACE2

Depositor

Lewis Cantley

Article

Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Promoter

EFS

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCD200

Plasmid

#164425

Purpose

AAV plasmid expressing full-length CD200 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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WT-β3

Plasmid

#166579

Purpose

For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

β3 (Itgb3 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-EIF2AK2-ts1

Plasmid

#174247

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (K93D) (P#580)

Plasmid

#14611

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) K93D (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K93D. Deficient in binding to CDK9. Inactive in 3…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pTET-IUPi

Plasmid

#122019

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pTET

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe RFP-DN-FOXO1-HA neo

Plasmid

#45813

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA and RFP

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pRK-ATF4 1-275

Plasmid

#26118

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa's 276-351 have been deleted

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only
pSAVED-CHAT_PCaspase_PCi_PC-σ

Plasmid

#214040

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-Pci-PC-σ

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Beclin-BATS

Plasmid

#30498

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Beclin, Barkor (BECN1, ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

Beclin 1 + 413-492 aa of Barkor

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pRK-ATF4 111-351

Plasmid

#26119

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF4 (ATF4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa's 1-110 have been deleted

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delZn1

Plasmid

#173933

Purpose

Bacterial expression of PARP1 lacking Zn1 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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