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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,051 results for: crispr grnas

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Showing: 5741 - 5760 of 14051 results

  5. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  6. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

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  12. Lenti sgRNA NGFR GFP out of frame

    Plasmid
    #155282
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280
    Depositor
    Martin Carroll
    Insert
    GFP out of frame IRES NGFR
    Available Since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155282-plasmid-map-sequence-id-310437

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  18. LentiCRISPRv2-ACTB-C4

    Plasmid
    #229849
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229849-plasmid-map-sequence-id-462154

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Showing: 5741 - 5760 of 14051 results