We narrowed to 81,643 results for: TRI

1. pEGFP-C3-PLK4-S305E-3xFLAG

   Plasmid

   #69840

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305E

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

   Plasmid

   #123346

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-CKAR
   

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

   Plasmid

   #69841

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S285A/T289A

   Promoter

   CMV

   Available Since

   Dec. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOXB4 (human) FLAG MSCV

   Plasmid

   #8528

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Missing N-terminal 14 amino acids; improved Ribos…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pCEP4-myc-HLA-A2-VN

   Plasmid

   #135485

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT-flk1-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80056

   Purpose

   Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   flk1

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

   Plasmid

   #129531

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-GFP-GFP-FLARE-AKAR

   Plasmid

   #123332

   Purpose

   Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   GFP-GFP-FLARE-AKAR
   

   Tags

   6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS2044

   Plasmid

   #79652

   Purpose

   ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

   Insert

   ssAAV Ple255-EmGFP WPRE
   

   Use

   AAV

   Promoter

   PAX6 HS234Z Enhancer

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS3-MT-BX/ARF1-64

    Plasmid

    #78762

    Purpose

    To overexpress ARF1-64 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    1-64

    Promoter

    CMV IE94

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCS3-MT-BX/ARF65-132

    Plasmid

    #78763

    Purpose

    To overexpress ARF65-132 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    65-132

    Promoter

    CMV IE94

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-SwitchON-sgCh2-2

    Plasmid

    #199637

    Purpose

    Tamoxifen-inducible expression of sgRNA control targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6P-1-INTS3-N

    Plasmid

    #128416

    Purpose

    Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal region (1–513 aa)

    Promoter

    tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HH06-LP Clone 6 heavy chain

    Plasmid

    #192176

    Purpose

    Clone 6 heavy chain (HH06)

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 6 heavy chain (HH06)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXB4 (human) HA-TAT-tag pET

    Plasmid

    #8526

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Tags

    HA

    Expression

    Bacterial

    Mutation

    replace T7 and HIS tags of pET with an HA tag;

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPKm-245

    Plasmid

    #90503

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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