We narrowed to 8,596 results for: dykddddk
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Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 V564F 3xFlag
Plasmid#110108Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
TagsFlagExpressionMammalianMutationmutated Valine 564 to Phenylalanine (V564F)PromoterCMVAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
TagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-A
Plasmid#35977DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "A" HIV cladeAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-SIVmac251
Plasmid#35979DepositorInserttransactivating protein
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationSIV strain mac251Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1_CR
Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant
Plasmid#106112PurposeInducible gene expression of FlagHA-PRKRA sgRNA PAM mutantDepositorInsertPRKRA (PRKRA Human)
TagsFlag and HAExpressionMammalianMutationsilent mutation of Gly32 (GGG-->GGT)Available SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-FMW-attB-AthHen1
Plasmid#104958PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 driversDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11d-FHis-DND1
Plasmid#70083PurposeBacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.DepositorInsertDnd1 (DND1 Human)
Tags6xHIS and FLAGExpressionBacterialMutationCodon optimized for bacterial expressionAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11d-FHis-DND1-Y102A
Plasmid#70082PurposeBacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.DepositorInsertDnd1 (DND1 Human)
Tags6xHIS and FLAGExpressionBacterialMutationY102A. Codon optimized for bacterial expressionAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101116PurposeEncodes dCas9(N) fused to LPAR1DepositorInsertLPAR1-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-Kaptin
Plasmid#100511PurposeExpresses FLAG tagged Kaptin in mammalian cells to make lentivirusDepositorAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagA(R37P)
Plasmid#99717PurposeMammalian expression of Flag-RagA(R37P)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2cl-FLAG
Plasmid#97077PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cellsDepositorAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A1-Ti
Plasmid#92018PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219V, C151AAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 V767A
Plasmid#86038Purposehuman VEGFR1 with V to A mutation at position 767DepositorInserthuman Flt1 V767A (FLT1 Human)
TagsFLAG, HA (please see depositor comments below), a…ExpressionMammalianMutationV767APromoterCMVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC_ZmSAE2-FLAG
Plasmid#91943PurposeBacterial expression of maize SAE2DepositorInsertZmSAE2-FLAG
TagsFLAGExpressionBacterialAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 Y1333F
Plasmid#86037Purposehuman VEGFR1 with Y to F mutation at position 1333DepositorInserthuman Flt1 Y1333F (FLT1 Human)
TagsFLAG, HA, and MycExpressionMammalianMutationY1333F; HA tag is inserted downstream of signal …PromoterCMVAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only