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PE-GsI-IIC-RT

Plasmid

#181808

Purpose

PE-GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-GsI-IIC-RT

Use

CRISPR

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Phleomycin

Plasmid

#188915

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Phleomycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBEVY-GA

Plasmid

#51227

Purpose

bi-directional expression vector for yeast, galactose inducible, ADE2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Hygromycin

Plasmid

#188929

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMN20

Plasmid

#11573

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Expression

Plant

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pMN19

Plasmid

#11572

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Expression

Plant

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pAM282

Plasmid

#226121

Purpose

Expression of cdc48

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 (CDC48 Budding Yeast)

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-URA

Plasmid

#188917

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEN-mSmo

Plasmid

#37673

Depositor

Philip Beachy

Article

Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

Insert

Smo (Smo Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pESC-URA-Ub-G76A-Rnq1-GFP

Plasmid

#21052

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP and ubiquitin

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pHES840

Plasmid

#89191

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWS926

Plasmid

#123052

Purpose

LexA bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexA-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Rab5a-7

Plasmid

#55444

Purpose

Localization: Ras GTPase, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Rab5a

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-HALO

Plasmid

#188943

Purpose

T7 expression vector for making his6- and HA3-tagged HALO protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-HALO

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-mCherry2Bx6

Plasmid

#105273

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCIP-GFP

Plasmid

#79012

Purpose

For mammalian expression of GFP.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

eGFP

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only