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We narrowed to 2,757 results for: fas

Showing: 1121 - 1160 of 2757 results

  2. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

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  6. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191097-plasmid-map-sequence-id-380121

    Enlarge

  7. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

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  8. pAAV-CAG-H2BmGreenLantern

    Plasmid
    #177332
    Purpose
    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.
    Depositor
    Gregory Petsko, Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B (Hist1h2bq Rat)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    This fusion protein was optimized to the Human co…
    Promoter
    CAG
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177332-plasmid-map-sequence-id-353323

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  9. pAAV-UbC-tdTomato

    Plasmid
    #62516
    Purpose
    Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures
    Depositor
    Pantelis Tsoulfas, Jae Lee
    Article
    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/
    Insert
    tdTomato
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbC
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62516-plasmid-map-sequence-id-107809

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  10. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

    Enlarge

  11. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid
    #191100
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mScarlet-I-Cdt1 (30-120)
    Use
    AAV and Synthetic Biology
    Tags
    mScarlet-I fused to residues 30-120 of human Cdt1…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191100-plasmid-map-sequence-id-380122

    Enlarge

  19. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  20. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

    Enlarge

  21. pdCas9-M-4A6

    Plasmid
    #73434
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4A6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73434-plasmid-map-sequence-id-135334

    Enlarge

  22. pdCas9-M-C4

    Plasmid
    #73427
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73427-plasmid-map-sequence-id-135327

    Enlarge

  23. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

    Enlarge

  24. pMEXneo-TrkC-Δ6

    Plasmid
    #190204
    Purpose
    To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.
    Insert
    TrkC (NTRK3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The extracellualr domain conatins only the signal…
    Promoter
    Moloney mouse sarcoma virus right LTR
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190204-plasmid-map-sequence-id-373714

    Enlarge

  25. pdCas9-M-4F2

    Plasmid
    #73431
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 4F2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73431-plasmid-map-sequence-id-135331

    Enlarge

  26. pdCas9-M-1B6

    Plasmid
    #73430
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1B6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73430-plasmid-map-sequence-id-139104

    Enlarge

  27. pdCas9-M-3H5

    Plasmid
    #73429
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3H5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73429-plasmid-map-sequence-id-135329

    Enlarge

  28. pdCas9-M-1D4

    Plasmid
    #73433
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1D4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73433-plasmid-map-sequence-id-135333

    Enlarge

  29. pdCas9-M-1E4

    Plasmid
    #73436
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 1E4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73436-plasmid-map-sequence-id-135335

    Enlarge

  30. pdCas9-M-5F5

    Plasmid
    #73432
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 5F5 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73432-plasmid-map-sequence-id-135332

    Enlarge

  31. pMEXneo-TrkC-TrkB-hybrid

    Plasmid
    #190205
    Purpose
    To express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.
    Insert
    TrkC (NTRK3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The second IgG like domain and the extracellular …
    Promoter
    Moloney mouse sarcoma virus right LTR
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190205-plasmid-map-sequence-id-374467

    Enlarge

  32. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

    Enlarge

  33. TFORF2091

    Plasmid
    #144382
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FANCD2 (FANCD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    144382-plasmid-map-sequence-id-431759

    Enlarge

  35. TFORF2090

    Plasmid
    #141916
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FANCD2 (FANCD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141916-plasmid-map-sequence-id-454923

    Enlarge

  38. pQhsCDtRNA+

    Plasmid
    #86014
    Purpose
    Multi-gene expression system for purification of human box C/D RNPs with U14-tRNA minigene
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14-tRNA chimera (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    LPP and Ptac
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86014-plasmid-map-sequence-id-169485

    Enlarge

  39. pQhsCDtRNA-

    Plasmid
    #86015
    Purpose
    Multi-gene expression system with human box C/D proteins with U14
    Depositor
    Hong Li
    Article
    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:
    Inserts
    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    Use
    Co-expression; purification
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    amino acids 1-401, amino acids 1-411, and amino a…
    Promoter
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86015-plasmid-map-sequence-id-169486

    Enlarge

  40. pCR4_miniFXN_7

    Plasmid
    #234360
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Article
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234360-plasmid-map-sequence-id-464761

    Enlarge

Showing: 1121 - 1160 of 2757 results