We narrowed to 23,531 results for: LEA
-
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-HA-MRE11-WT
Plasmid#82033PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRE11 (MRE11 Human)
TagsHAExpressionMammalianPromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDW1775
Plasmid#13456PurposePlant ZFN expression vector.DepositorTypeEmpty backboneTagsAcV5ExpressionPlantAvailable sinceDec. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Tag2B EDD
Plasmid#37188DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEDD (UBR5 Human)
Tags6x His Tag and FLAGExpressionMammalianMutationPlease see Depositor CommentsPromoterCMVAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 NusA TEV LIC cloning vector (1N)
Plasmid#29657DepositorTypeEmpty backboneTags6xHis, NusA, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and Thrombin cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pMBP-AsCas12a
Plasmid#113430PurposeExpresses 10xHis-MBP fused to AsCas12aDepositorInsertAsCas12a
TagsMBPExpressionBacterialPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcdna nesprin TS
Plasmid#68127Purposenesprin tension sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmouse nesprin mini 2G (Syne2 Mouse)
ExpressionMammalianPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV Flag WT-PTEN
Plasmid#22231DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPTEN (PTEN Human)
TagsFlagExpressionMammalianAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OCT4-eGFP-PGK-Puro
Plasmid#31937PurposeOct4 targeting donor vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOCT4 (POU5F1 Human)
UseTargeting donorTagseGFPAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Emerin pEGFP-C1 (637)
Plasmid#61993Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEmerin (EMD Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ825
Plasmid#39315DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A)PromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLDLR (LDLR Human)
TagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas serviceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianPromoterEf1aAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
-
BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-mCherry-IRES-Flpo
Plasmid#55634PurposeExpresses Flpo in Mammalian CellsDepositorHas serviceAAV1 and AAV RetrogradeInsertsmCherry
Flpo
UseAAVExpressionMammalianPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSP469
Plasmid#65771PurposeHuman expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by