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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,492 results for: PUC19

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Showing: 561 - 580 of 2492 results

  2. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

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  5. AICSDP-1:PXN-EGFP

    Plasmid
    #87420
    Purpose
    Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    PXN Homology Arms with linker-EGFP (PXN Human)
    Use
    CRISPR; Donor template
    Tags
    linker-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87420-plasmid-map-sequence-id-173974

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  6. pSBbi-BH

    Plasmid
    #60515
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60515-plasmid-map-sequence-id-97531

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  9. pAAVS1-shRNA

    Plasmid
    #82697
    Purpose
    For targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.
    Depositor
    Alan Mullen
    Article
    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:
    Type
    Empty backbone
    Use
    RNAi
    Promoter
    hPGK, U6
    Available Since
    Nov. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    82697-plasmid-map-sequence-id-157348

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  10. pT2ST

    Plasmid
    #59384
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-dfrA
    Use
    Template
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59384-plasmid-map-sequence-id-92128

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  11. pMU131

    Plasmid
    #66811
    Purpose
    Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI
    Depositor
    Lee Lynd
    Article
    Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66811-plasmid-map-sequence-id-117172

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  18. pSBtet-BH

    Plasmid
    #60499
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60499-plasmid-map-sequence-id-97548

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  20. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

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Showing: 561 - 580 of 2492 results