We narrowed to 23,148 results for: REP

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pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

Plasmid

#87906

Purpose

sgRNA targeting GFP

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgGFP2, sgGFP3

Expression

Mammalian

Promoter

H1, U6, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-zic2a-cytoBirA-2A-mCherry_Ras

Plasmid

#80064

Purpose

Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Zic2a enhancer driving c-fos promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMTB2-Avi-Cerulean-Rpl10a

Plasmid

#79880

Purpose

Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)

Tags

Avi

Expression

Bacterial

Promoter

Beta-actin2

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pKlab003-pcDNA3.1-FKBP5-3xFLAG

Plasmid

#249006

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged FKBP5 protein sequence for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

FKBP5 (FKBP5 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_cmlc2(myl7)_cmlc2-nmKate

Plasmid

#238374

Purpose

Drives expression of 3 different gRNAs targeting cmlc2 (myl7), and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting cmlc2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP4-SEP

Plasmid

#174406

Purpose

Reporter for VAMP4 exocytosis in mammalian cells

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (VAMP4 Human)

Tags

superecliptic phulorin (mutated GFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSBLego

Plasmid

#219967

Purpose

The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

Depositor

Markus Seeger

Article

Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

Type

Empty backbone

Tags

SLEHHHHHH and pelB signal sequence

Expression

Bacterial

Promoter

pBAD

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-MLC2 WT

Plasmid

#195235

Purpose

Mammalian expression of GFP tagged MLC2. Wild-type

Depositor

David Bryant, Keith Mostov

Article

Yu et al EMBO Rep. 2008 Sep;9(9):923-9. doi: 10.1

Insert

MLC2 (MYL2 Human)

Expression

Mammalian

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 +KTS N terminal HA

Plasmid

#217639

Purpose

Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 +KTS isoform (WT1 Human)

Use

Gateway Cloning

Tags

HA

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-WT1 -KTS N terminal HA

Plasmid

#217641

Purpose

Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning

Depositor

Constanze Bonifer

Article

Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

Insert

WT1 -KTS isoform (WT1 Human)

Use

Gateway Cloning

Tags

HA

Mutation

p.408_410delKTS

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-TfCut2-S121P-D174S-D204P

Plasmid

#218695

Purpose

Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TfCut2

Tags

10xHis

Expression

Bacterial

Mutation

S121P-D174S-D204P

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-BhrPETase

Plasmid

#218692

Purpose

Encodes codon optimized BhrPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

BhrPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Sci Rep. 2024 Mar 19;14(1):6541. doi: 10

Insert

iSeroSnFR_cytosolic

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C88S-FLAG

Plasmid

#198187

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C88S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-StefinA

Plasmid

#182404

Purpose

Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DARPinOFF7

Plasmid

#182396

Purpose

Bacterial expression of DARPinOFF7

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DARPinOFF7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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