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pYPQ166-OsPE2

Plasmid

#141080

Purpose

Gateway entry clone for CRISPR-Cas9(H840A) prime editing

Depositor

Yiping Qi

Article

Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

Insert

zCas9(H840A)-OsM-MLV-RT

Use

CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

Tags

3x FLAG, NLS and NLS

Expression

Plant

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTphiC31

Plasmid

#127518

Purpose

Plasmid encodes H. sapiens codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-ProC3-Cre/mCherry

Plasmid

#126006

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

Cre-2A-mCherry

Use

AAV

Promoter

ProC3

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Src

Plasmid

#118308

Purpose

Expression of mouse Src

Depositor

Philip Stork

Article

Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

Insert

Src (Src Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-HLA-A2_GFP

Plasmid

#85161

Purpose

HLA.A2_GFP fusion with 2 glycines in between

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Gateway Cloning

Tags

EGFP

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-pHlash

Plasmid

#61552

Purpose

Expression of pHlash in mammalian cells

Depositor

Carl Johnson

Article

Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

Insert

pHlash

Tags

NA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pENTR11 mSTIM2 myc

Plasmid

#17734

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM2 (Stim2 Mouse)

Tags

Myc

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
lentiBE3-SpRY-Blast

Plasmid

#199303

Purpose

Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

FNLS-BE3-SpRY

Use

CRISPR and Lentiviral

Tags

2A tag

Expression

Mammalian

Promoter

EFS

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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co luxE pcDNA3.1(+)

Plasmid

#129089

Purpose

Expression of luxE in mammalian cells

Depositor

Stefan Hell

Article

Gregor et al Proc Natl Acad Sci U S A. 2019 Dec 2. pi

Insert

co luxE

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-1x

Plasmid

#124679

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLX302-MKP5-V5 puro

Plasmid

#87770

Purpose

Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP5 (DUSP10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDB22

Plasmid

#61342

Purpose

Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-cMyc1A NLS and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJC53.2/PRMT5

Plasmid

#35692

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2012 Mar;139(6):1083-94. Ep

Insert

Protein arginine methyltransferase 5

Promoter

Sp6 for sense, T7 for sense and antisense

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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myc-Rapr-FAK

Plasmid

#25926

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HK3

Plasmid

#23731

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK3 (HK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
p1303 DV-SFTPC-tdTomato

Plasmid

#126696

Purpose

donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

tdTomato

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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