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scAAV-Cre

Plasmid

#177933

Purpose

Expresses Cre-recombinase and can be packaged into AAV particles.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Insert

CAG-Cre

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AiP20142 - pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA

Plasmid

#230803

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP39

Plasmid

#22581

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP39 (USP39 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C5_15

Plasmid

#60322

Purpose

This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

Non active element (nearest TSS PHLDA1) (PHLDA1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC30A1

Plasmid

#132137

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A1 (SLC30A1 Human)

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125691

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff DREADD Gq-mCherry

Plasmid

#183533

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA-SV40-puro

Plasmid

#240539

Purpose

Lentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFREE019Env

Plasmid

#137670

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein (env HIV-1)

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CDKN2A-L78Hfs*41

Plasmid

#116222

Purpose

Lentiviral expression of CDKN2A L78Hfs*41

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDKN2A (CDKN2A Human)

Use

Lentiviral

Mutation

L78Hfs*41

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR2B

Plasmid

#17925

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

N-methyl D-aspartate receptor 2B (Grin2b Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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mAzurite-16xRhoBAST

Plasmid

#196347

Purpose

Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

mAzurite-16xRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Expression

Bacterial

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-80L iLID (C530M)

Plasmid

#60408

Purpose

E. coli expression of iLID (C530M)

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

iLID C530M

Tags

His

Expression

Bacterial

Mutation

C530M

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
Venus-2xFYVE

Plasmid

#234746

Purpose

Mammalian expression of endosome marker Venus-2xFYVE

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

Venus-2xFYVE

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb-eGFP + RUBY

Plasmid

#233310

Purpose

GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

Ubi:ccdb-eGFP + RUBY

Use

Gateway destination

Tags

eGFP

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-iCasp9

Plasmid

#199441

Purpose

Express inducible Caspase9 in mammalian cells

Depositor

Chao Tang

Article

Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

Insert

inducible Caspase9 (CASP9 Human)

Use

Lentiviral

Tags

DmrB and mCherry

Expression

Bacterial and Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pP2A-mCherry-N1

Plasmid

#84329

Purpose

Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

P2A-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pX330-RPA194

Plasmid

#247352

Purpose

Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_cpCys203

Plasmid

#247759

Purpose

mammalian expression of the cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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