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We narrowed to 8,939 results for: pcDNA3

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Showing: 5861 - 5880 of 8939 results

  2. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  3. BIC-Gag-CAS9

    Plasmid
    #119942
    Purpose
    encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.
    Depositor
    Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci
    Article
    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10
    Insert
    GAG FMLV fused to spCAS9
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    hCMV
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119942-plasmid-map-sequence-id-235075

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  7. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  9. Dronpa-FHA1

    Plasmid
    #87711
    Purpose
    Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Dronpa-FHA1
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87711-plasmid-map-sequence-id-176141

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  13. p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)

    Plasmid
    #52216
    Purpose
    mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation
    Depositor
    Eric Huang
    Article
    Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2
    Insert
    HIF1alpha (401delta603) LCLL (HIF1A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    aa 401-603 deleted, removes the oxygen-dependent …
    Promoter
    CMV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. Lyn-FLINC-EKAR1

    Plasmid
    #87707
    Purpose
    Fluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87707-plasmid-map-sequence-id-171091

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  15. Lyn-FLINC-EKAR1(TA)

    Plasmid
    #87708
    Purpose
    Negative-control mutant of Lyn-FLINC-EKAR1.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Lyn-FLINC-EKAR1(TA)
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87708-plasmid-map-sequence-id-171092

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  16. pUB2.1

    Plasmid
    #40728
    Purpose
    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background
    Depositor
    Thomas Hughes
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40728-plasmid-map-sequence-id-128586

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  17. pcmCherry 3.1(-)

    Plasmid
    #62803
    Purpose
    Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry
    Depositor
    Michal Minczuk
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    62803-plasmid-map-sequence-id-103515

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  18. pEZYmyc-His

    Plasmid
    #18701
    Purpose
    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Tags
    Myc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18701-plasmid-map-sequence-id-181026

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  20. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

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Showing: 5861 - 5880 of 8939 results