We narrowed to 18,061 results for: Mut

1. pcDNA3-BaLgp120-8his

   Plasmid

   #100925

   Purpose

   Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Use

   Tags

   CD5 leader sequence and Purification tag after K5…

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TDP-43_CTD_Q331K

   Plasmid

   #98673

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Q331K

   Promoter

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-GFPLaminA-L530P

   Plasmid

   #32710

   Depositor

   Wendy Bickmore

   Article

   Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L530P

   Promoter

   CMV

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-PKM2-R399E

   Plasmid

   #122691

   Purpose

   Express His-tagged PKM2 (R399E) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   R399E

   Promoter

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZS*12-MS2-GFP

   Plasmid

   #26245

   Depositor

   Philippe Cluzel

   Article

   Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

   Insert

   MS2-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

   Promoter

   Available Since

   Nov. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. VCP(A232E)-EGFP

   Plasmid

   #23973

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Use

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   A232E

   Promoter

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Rab21-CCSS

   Plasmid

   #83424

   Purpose

   Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

   Promoter

   CMV

   Available Since

   Nov. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP C3-Lats1

   Plasmid

   #19053

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1, N mutant (LATS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   **

   Promoter

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-AID-BLM-UGI

    Plasmid

    #221218

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-BLM-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM4891 (pCV0003)

    Plasmid

    #120080

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Tags

    Expression

    Mutation

    mobA Y25F

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR_L1-Turbo-YFP-NLS-STOP-L2

    Plasmid

    #127361

    Purpose

    gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW212

    Plasmid

    #172196

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Expression

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HsGRK6-mVenus

    Plasmid

    #137780

    Purpose

    Visualization of GRK6

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Human GRK6 (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-myc-Miro1 P13V

    Plasmid

    #47889

    Purpose

    Expresses myc tagged Miro1 P13V constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 P13V (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    P13V, constitutively active mutant

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLKO-puro-tetON-shRFP

    Plasmid

    #110940

    Purpose

    Tet-inducible shRNA targeting RFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shRFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiV_Neo_SIK3_CR

    Plasmid

    #108104

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation)

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHHM.X513-iNicSnFR3b-(V7)

    Plasmid

    #124881

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)
    

    Use

    Tags

    HA Tag, His Tag, and NA

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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