We narrowed to 28,464 results for: Tat

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GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

Plasmid

#165026

Purpose

Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

Plasmid

#165028

Purpose

Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Vimentin-DiB-RM

Plasmid

#168783

Purpose

Vimentin - DiB-RM fusion protein

Depositor

Jens Meiler

Article

Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

Insert

Vimentin-DiB-RM

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-CFP-FRB1C_no-linker

Plasmid

#162434

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-CFP-FRB1C_no_linker

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 FL-H1

Plasmid

#163583

Purpose

express Dcp2 FL-H1 at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

The first HLM (EDQLKSYAEEQLKLLLGITKEE) is added t…

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔN

Plasmid

#163575

Purpose

express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of N-terminal domain (1-242) of Dcp2

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300

Plasmid

#163577

Purpose

express Dcp2 300 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domian (301-970)

Promoter

DCP2 promoter

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Lyn-YFP-Clover-FRB1N

Plasmid

#162439

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-Clover-FRB1N

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1

Plasmid

#160988

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2-2A2

Plasmid

#160991

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N2

Plasmid

#160989

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1-2A2

Plasmid

#160990

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-S

Plasmid

#136388

Purpose

Expresses NERD-S static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-S

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39I-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414 - NERD-C

Plasmid

#136389

Purpose

Expresses NERD-C static protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

NERD-C

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-F30L-V39I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNAmreB

Plasmid

#149634

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_Psyn-sgRNAmreB

Plasmid

#149566

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050I (notch)

Plasmid

#132421

Purpose

express arrays of gRNA targeting Notch under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

notch gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1050V (wingless)

Plasmid

#132424

Purpose

express arrays of gRNA targeting Wingless under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

wingless gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-3'UTR

Plasmid

#136052

Purpose

REG1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (ATTGTATCTCTGTAGTTTAAG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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