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pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

Plasmid

#229814

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-SMARCA4

Plasmid

#65391

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA4 (SMARCA4 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.GPI

Plasmid

#178331

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-APP-mGFP

Plasmid

#196704

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mCherry and mEGFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-HOXA9

Plasmid

#237639

Purpose

For overexpression of mEGFP-NUP98-HOXA9

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-HOXA9 (NUP98, HOXA9 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 R62D actin-3XNLS P2A mCherry

Plasmid

#58477

Purpose

Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Mutation

Changed Arginine 62 to Aspartic Acid

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.GPI

Plasmid

#178335

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1h

Plasmid

#113050

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Lamin A wildtype

Plasmid

#118709

Purpose

Lentiviral TET-ON inducible GFP-Lamin A wildtype

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Lamin A human wildtype (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mCitrine-DNAJC5 delL116

Plasmid

#246734

Purpose

Mammalian expression of human DNAJC5 L116 deleted with a mCitrine (YFP) tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

mCitrine

Expression

Mammalian

Mutation

delL116

Promoter

CMV

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-TAF1

Plasmid

#65395

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TAF1 (TAF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.PDGFR

Plasmid

#178333

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NUP98-DDX10

Plasmid

#237640

Purpose

For overexpression of mEGFP-NUP98-DDX10

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NUP98-DDX10 (NUP98, DDX10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-APPP1-mGFP

Plasmid

#196705

Purpose

As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mCherry and mEGFP

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin C661S

Plasmid

#118711

Purpose

Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin C661S (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

C661S farnesylation mutant

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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