We narrowed to 7,109 results for: mag

1. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

   Plasmid

   #87109

   Purpose

   BPNLS-Cas9-BPNLS-2AGFP expression plasmid

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   1BPNLS-Cas9-1BPNLS-2A-EGFP
   

   Use

   CRISPR

   Tags

   3xFLAG (on Cas9)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-45b-mCherry-Cbx5

   Plasmid

   #194547

   Purpose

   Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   Cbx9 (HP1a)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-mEGFP-PHOX2B-WT

   Plasmid

   #194576

   Purpose

   Mammalian Expression of mEGFP-PHOX2B WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   PHOX2B (PHOX2B Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

   Plasmid

   #187844

   Purpose

   Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Tags

   Ig k-chain leader sequence, SNAPf-tag, and meGFPe…

   Expression

   Mammalian

   Mutation

   meGFPe: asparagine 198 to aspartic acid and tyros…

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-mEGFP-PHOX2B-MUT

   Plasmid

   #194577

   Purpose

   Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   PHOX2B (PHOX2B Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   S207Afs*102

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-mEGFP-RAX-WT

   Plasmid

   #194572

   Purpose

   Mammalian Expression of mEGFP-RAX WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   RAX (RAX Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104493

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TdLanYFP-AURKA-Aquamarine

   Plasmid

   #228562

   Purpose

   Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Tags

   Aquamarine and TdLanYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. G protein alpha-s-mCherry

    Plasmid

    #66968

    Purpose

    G protein alpha-s internally tagged with mCherry and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCherry (Gnas Rat, Discosoma sp.)
    

    Tags

    internal EE epitope (residues 189-194 in alpha- s…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCjedCas9

    Plasmid

    #172216

    Purpose

    Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

    Depositor

    John van der Oost

    Article

    Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

    Insert

    catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
    

    Tags

    Histag

    Mutation

    changed Asp 8 to Ala and His 559 to Ala

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid

    #119188

    Purpose

    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid

    #192785

    Purpose

    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Tags

    Ig k-chain leader sequence, SNAPf-tag, and mXFPe

    Expression

    Mammalian

    Mutation

    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

    Plasmid

    #74306

    Purpose

    Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Inserts

    TRPV4 (Trpv4 Rat)
    ferritin (FTL and HTL1) (FTL, FTH1 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG and p2A

    Expression

    Mammalian

    Promoter

    CMV (after cre recombination)

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-mEGFP-RAX-MUT

    Plasmid

    #194573

    Purpose

    Mammalian Expression of mEGFP-RAX Mutant (S222Rfs*63) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    RAX (RAX Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    S222Rfs*63

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-mEGFP-MYOD1-MUT

    Plasmid

    #194571

    Purpose

    Mammalian Expression of mEGFP-MYOD1 Mutant (R188Pfs*90) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MYOD1 (MYOD1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    R188Pfs*90

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-AlbEx14-mNG

    Plasmid

    #201780

    Purpose

    knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

    Depositor

    Hajime Hirase

    Article

    Vittani et al Fluorescence Imaging of the Brain. Neuro

    Inserts

    Alb gRNA (Alb Mouse)
    Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
    

    Use

    AAV and CRISPR

    Promoter

    U6

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid

    #158758

    Purpose

    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    nullCoChR-GCaMP6f-Kv2.1
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    synapsin promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. G protein alpha-s-mCerulean

    Plasmid

    #66084

    Purpose

    G protein alpha-s internally tagged with mCerulean and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid

    #118717

    Purpose

    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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