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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAM005

Plasmid

#170009

Purpose

Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PvirK

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pL2-G(P)7G

Plasmid

#137100

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(P)7G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G2

Plasmid

#137091

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GG

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G3

Plasmid

#137092

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt10a

Plasmid

#42288

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt10a (Wnt10a Mouse)

Expression

Mammalian

Available Since

June 21, 2013

Availability

Academic Institutions and Nonprofits only
pKB226

Plasmid

#170016

Purpose

Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Defensin HNP-1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBLO44.1_dProtease

Plasmid

#121631

Purpose

CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

TEV protease

Expression

Mammalian

Promoter

U6 and chicken beta-actin promoter

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-C-SF-TAP-DEST-TLK1-WT

Plasmid

#218829

Purpose

Expresses C-terminal Strep-FLAG-tagged wild type human TLK1 variant 5 in a retrovirus vector.

Depositor

Travis Stracker

Insert

TLK1 (TLK1 Human)

Tags

FLAG-STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 promoter

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAM002

Plasmid

#170006

Purpose

Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PpmrD

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CasRx gRNA targeting EZH2

Plasmid

#219655

Purpose

hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

CasRx gRNA targeting EZH2

Use

CRISPR

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFD-FKBP12

Plasmid

#137108

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FKBP12

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBA2257

Plasmid

#38151

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

MBP-proMeCPA fusion protein

Tags

maltose binding protein

Expression

Bacterial

Promoter

tac

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NASP-Halo

Plasmid

#175340

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NASP (NASP Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNSA-FLUC

Plasmid

#98143

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, zeocin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Tags

CAAX and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-GFP-CENPBpA

Plasmid

#120162

Purpose

EGFP followed by the poly(A) site from human CENPB gene

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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