We narrowed to 14,350 results for: cas9

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pSG

Plasmid

#169416

Purpose

Used for guide cloning

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Type

Empty backbone

Use

Other

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS252

Plasmid

#73720

Purpose

SapTrap GFP + Cbr-unc-119 donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMLS279

Plasmid

#73729

Purpose

SapTrap FLP-on C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

C-terminal FLP-on

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LRG2.1-CyOFP1

Plasmid

#124771

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

Depositor

Jason Sheltzer

Publication

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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LRG2.1-mOrange

Plasmid

#124772

Purpose

3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

Depositor

Jason Sheltzer

Publication

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_S139A-GS3

Plasmid

#204749

Purpose

Expression of Cas9 and human H2AX with the S139A mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX S139A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the S139A mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCB32

Plasmid

#111911

Purpose

Expresses gRNA to target Nte1 locus and has HygR marker

Depositor

Yi Tang

Insert

gRNA cassette targeting Nte1 locus

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

SNR52p

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPN10-gCAG

Plasmid

#114384

Purpose

pPN10 with gRNA for spCRISPR-Cas9 targeted to CAG repeat, PAM: CAG

Depositor

Vincent Dion

Publication

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gCAG

Use

CRISPR

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65-RTA(75-190)

Plasmid

#99670

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Publication

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-p65-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRB1084

Plasmid

#71516

Purpose

Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGMC00023 (aka pRC0434)

Plasmid

#195309

Purpose

Lentiviral expression vector derived from LEGO-G2 with G418/Neomycin selection

Depositor

Raj Chari

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLV/eGFP-U6-sgRNA

Plasmid

#170544

Purpose

A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

Depositor

Jacob Giehm Mikkelsen

Publication

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-puro U6 sgRNA Oct4A -158

Plasmid

#50922

Purpose

U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Oct4A -158 sgRNA (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAEF5

Plasmid

#136305

Purpose

Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

Depositor

Gilles Fischer

Publication

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Type

Empty backbone

Use

CRISPR

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Publication

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Expression

Bacterial and Yeast

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Level 1 P4 TaU6 guide acceptor

Plasmid

#165600

Purpose

GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

TaU6 promoter::LacZ::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Sgrna acceptor with…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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