We narrowed to 8,708 results for: sgRNA

1. pCR8-attL5-pegRNA-attR3

   Plasmid

   #213054

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpyB

   Plasmid

   #220186

   Purpose

   sgRNA expression vector - pBG35 promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   pBG35 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBG35

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

4. PlayBack-U6-Xba1

   Plasmid

   #203300

   Purpose

   For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTJV1ScKm-rpoB

   Plasmid

   #166980

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   NeoR/KanR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330 SUZ12 - pDY083

   Plasmid

   #171109

   Purpose

   Expresses Cas9 and an sgRNA targeting SUZ12

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgPal7 (p2Tol-U6-sgPal7-HygR)

   Plasmid

   #71484

   Purpose

   Expresses palindromic sgRNA for use in self-cloning CRISPR

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   sgPal7
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB70

   Plasmid

   #47943

   Purpose

   Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330 EZH2 - pDY052

   Plasmid

   #171107

   Purpose

   Expresses Cas9 and an sgRNA targeting EZH2

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GEARBOCS-SPARCL1-GeneTRAP

    Plasmid

    #218184

    Purpose

    To KO and genetrap mouse Hevin simultaneously

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GEARBOCS-SPARCL1 KO

    Plasmid

    #218182

    Purpose

    To KO Hevin in astrocytes

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBC2103-reci

    Plasmid

    #117172

    Purpose

    U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_arCas9 without NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAN-PA4-RFP

    Plasmid

    #62250

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA4

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB60

    Plasmid

    #47941

    Purpose

    Can be used to generate synthetic guide in vitro from T7 promotor

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    synthetic guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJF131B

    Plasmid

    #113326

    Purpose

    pTet-W108 scRNA.b2, pTet-RR2-sgRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2 and sgRNA_RR2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAN-PA2-RFP

    Plasmid

    #62248

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA2

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. YN2_1_IL50_HOlocus

    Plasmid

    #194426

    Purpose

    Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    NA

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6>XX

    Plasmid

    #87542

    Purpose

    Component 1 of 2 for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgPal1 (p2Tol-U6-sgPal1-HygR)

    Plasmid

    #71483

    Purpose

    Expresses palindromic sgRNA for use in self-cloning CRISPR

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Insert

    sgPal1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Modified 7SK

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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